All Phenotypes Tnk2<tm1.2Npma> MGI Mouse

increased effector memory CD8-positive, alpha-beta T cell number ( J:341740 )

• percentages of CD44^hiCD62L^low memory CD8+ T cells and CD137+ effector CD8+ T cells are increased in the spleen

• however, no differences are seen in the ratios of FoxP3-CD4+ helper T cells, Foxp3+CD4+ regulatory T cells, CD8+ CTLS, CD19+ B cells, NK1.1+ NK cells, and CD11b Gr-1+ myeloid cells

• spleens from male mice injected with TRAMP-C2 tumor cells show an increase in the number of effector CD44^hiCD62L^low CD8+ T cells, CD137+/CD8+ cytotoxic T cells and CD69+/CD4+ cells

abnormal regulatory T cell number ( J:341740 )

• mice injected with TRAMP-C2 tumor cells show an increase in CD4+CD69+ cells expressing perforin (subset of regulatory T cells involved in tumor-induced immunosuppresion) but a decrease in the number of T regulatory cells compared to controls

increased activated T cell number ( J:341740 )

• mice exhibit spontaneous activation of CD8+ and CD4+ T cells

• however, mice show no differences in alanine transaminase levels or anti-nuclear antibodies and minimal signs of inflammation or fibrosis in liver, kidney, small intestine, or lungs indicating that the activated T-cell response does not lead to a hyperactive response or generate autoimmune disease

• lymph nodes from male mice injected with TRAMP-C2 cells exhibit an increase in the number of CD4+ and CD8+ cells, indicating increased T cell activation

abnormal T cell physiology ( J:341740 )

• splenocytes (basal T cells) exhibit an increased calcium response and an increase in calcium flux upon anti-CD3 antibody treatment indicating faster and larger calcium responses from T cells

• splenocytes incubated with TRAMP-C2 prostate adenocarcinoma cells exhibit higher calcium responses

increased interferon level ( J:341740 )

• increase in splenocyte IFN-gamma levels

• splenocytes from male mice injected with TRAMP-C2 tumor cells express increased levels of IFN-gamma

increased circulating interferon-gamma level ( J:341740 )

• increase in blood and splenocyte IFN-gamma levels

increased interleukin level ( J:341740 )

• increase in splenocyte IL-2 levels

• splenocytes from male mice injected with TRAMP-C2 tumor cells express increased levels of IL-2

increased circulating interleukin-2 level ( J:341740 )

• increase in blood IL-2 levels

decreased tumor growth/size ( J:341740 )

• males injected subcutaneously with TRAMP-C2 tumor cells exhibit a reduction in tumor growth, with only 3 of 10 mice developing tumors and no trace of tumor in the remaining mice

• mice challenged with B16 melanoma cells show a decrease in the volume of B16 tumors

• mice injected with anti-CD4, anti-CD8, or both types of depletion antibodies to deplete T cells and then implanted with TRAMP-C2 tumor cells exhibit inhibited tumor growth

• adoptive transfer of purified T cells from mutant mice into TRAMP-C2-implanted NSG mice results in significant tumor growth regression