Phenotypes associated with this allele

• Allele Symbol: Alkal2^tm1Schl
• Allele Name: targeted mutation 1, Joseph Schlessinger
• Allele ID: MGI:7578836
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Alkal2^tm1Schl/Alkal2^tm1Schl	involves: C57BL/6	MGI:7660934
cx2	Alkal1^tm1Schl/Alkal1^tm1Schl Alkal2^tm1Schl/Alkal2^tm1Schl	involves: C57BL/6	MGI:7660939
cx3	Alkal1^tm1Schl/Alkal1^+ Alkal2^tm1Schl/Alkal2^tm1Schl	involves: C57BL/6	MGI:7660943
cx4	Alkal1^tm1Schl/Alkal1^tm1Schl Alkal2^tm1Schl/Alkal2^+	involves: C57BL/6	MGI:7660944

Genotype
MGI:7660934

hm1

• Allelic Composition: Alkal2^tm1Schl/Alkal2^tm1Schl
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased lean body mass ( J:324631 )

• mice are significantly leaner than wild-type or heterozygous controls from the age of 14 weeks onward

decreased body weight ( J:324631 )

• mice fed a standard chow diet show a significantly lower body weight than wild-type or heterozygous controls at 36 and 52 weeks of age

slow postnatal weight gain ( J:324631 )

• male and female mice fed a standard chow diet gain less weight than wild-type or heterozygous controls from the age of 4 weeks onward

thin body ( J:324631 )

• mice fed a standard chow diet are thin, showing a marked reduction in their circumference but no change in body length

behavior/neurological

decreased fluid intake ( J:324631 )

• after a 16h fast, mice show a small but significant decrease in water intake upon refeeding

increased fluid intake ( J:324631 )

• when food deprived for 16 h, mice show a significant increase in water intake during the dark phase of the fasting period

decreased compensatory feeding amount ( J:324631 )

• after a 16h fast, mice show a small but significant decrease in food intake upon refeeding

hyperactivity ( J:324631 )

• 3- to 6-mo-old mice fed a standard chow diet are slightly more active during daytime but show a significantly higher physical activity during nighttime

hyperactivity elicited by feed restriction ( J:324631 )

• when food deprived for 16 h, mice exhibit a trend towards increased activity during the dark phase of the fasting period

adipose tissue

decreased subcutaneous adipose tissue amount ( J:324631 )

• subcutaneous WAT weight is significantly lower than in wild-type controls

decreased white adipose tissue amount ( J:324631 )

• mice exhibit a significant reduction in the size of white adipose tissue (WAT) depots

• however, the weight of fat mass from brown adipose tissue (BAT) is not significantly altered

decreased white fat cell size ( J:324631 )

• mice exhibit a higher percentage of small adipocytes in gonadal, retroperitoneal and subcutaneous WAT

decreased gonadal fat pad weight ( J:324631 )

• gonadal WAT weight is significantly lower than in wild-type controls

decreased retroperitoneal fat pad weight ( J:324631 )

• retroperitoneal WAT weight is significantly lower than in wild-type controls

decreased percent body fat/body weight ( J:324631 )

• adult mice fed a standard chow diet show a significant decrease in overall body fat with respect to overall body weight

browned white adipose tissue morphology ( J:324631 )

• qRT-PCR analysis showed increased expression levels of Ucp1, Ppargc1a, Cidea, and Adrb3 in WAT depots, suggesting some browning of WAT

homeostasis/metabolism

increased noradrenaline level ( J:324631 )

• mice exhibit a significant increase of norepinephrine levels in subcutaneous WAT

increased energy expenditure ( J:324631 )

• when food deprived for 16 h, mice exhibit significantly higher energy expenditure (EE) during the dark phases of the fasting period

• 3- to 6-mo-old mice fed a standard chow diet show significantly increased EE during nighttime; total EE is also significantly increased

increased carbon dioxide production ( J:324631 )

• 3- to 6-mo-old mice fed a standard chow diet show a significantly greater CO₂ production during nighttime; total CO₂ production is also significantly increased

increased oxygen consumption ( J:324631 )

• 3- to 6-mo-old mice fed a standard chow diet show a significantly greater oxygen consumption during nighttime; total oxygen consumption is also significantly increased

increased respiratory quotient ( J:324631 )

• when food deprived for 16 h, mice exhibit a significantly higher respiratory exchange rate (RER) due to increased CO₂ production during the dark phases of the fasting period

• 3- to 6-mo-old mice fed a standard chow diet show a significantly higher RER during nighttime

improved glucose tolerance ( J:324631 )

• 3- to 6-mo-old mice fed a standard chow diet show improved glucose tolerance

increased insulin sensitivity ( J:324631 )

• 3- to 6-mo-old mice fed a standard chow diet show increased sensitivity to insulin

integument

decreased subcutaneous adipose tissue amount ( J:324631 )

• subcutaneous WAT weight is significantly lower than in wild-type controls

Genotype
MGI:7660939

cx2

• Allelic Composition: Alkal1^tm1Schl/Alkal1^tm1Schl; Alkal2^tm1Schl/Alkal2^tm1Schl
• Genetic Background: involves: C57BL/6

growth/size/body

decreased body weight ( J:324631 )

• double knockout mice fed a standard chow diet show a significantly lower body weight than double heterozygous controls at 26 weeks of age

slow postnatal weight gain ( J:324631 )

• double knockout mice fed a standard chow diet gain significantly less weight than double heterozygous controls

Genotype
MGI:7660943

cx3

• Allelic Composition: Alkal1^tm1Schl/Alkal1⁺; Alkal2^tm1Schl/Alkal2^tm1Schl
• Genetic Background: involves: C57BL/6

growth/size/body

decreased body weight ( J:324631 )

• mice fed a standard chow diet show a significantly lower body weight than double heterozygous controls at 26 weeks of age

slow postnatal weight gain ( J:324631 )

• mice fed a standard chow diet gain significantly less weight than double heterozygous controls, with body weight kinetics similar to those in seen in double knockout mice

Genotype
MGI:7660944

cx4

• Allelic Composition: Alkal1^tm1Schl/Alkal1^tm1Schl; Alkal2^tm1Schl/Alkal2⁺
• Genetic Background: involves: C57BL/6

growth/size/body

growth/size/body region phenotype ( J:324631 )

N • mice fed a standard chow diet show no differences in body weight kinetics relative to double heterozygous controls, indicating that Alkal2 deficiency causes thinness whereas Alkal1 deficiency does not