Analysis Tools
growth/size/body
|
• HFD-fed mice exhibit significantly lower fat mass than diet-matched wild-type controls
• however, lean body mass and food intake are relatively normal after HFD feeding
|
|
• after high-fat diet (HFD) feeding for 7 wks, both sexes exhibit significantly lower body weights than diet-matched wild-type controls
• however, body weight is normal under normal chow diet (NCD) feeding
|
|
• mice are protected from HFD-induced obesity with significantly lower body weight gain, white adipose tissue (WAT) mass and WAT depot weights, smaller white adipocyte size, and less crown-like structures and inflammation in WAT than diet-matched wild-type controls
|
adipose tissue
|
• HFD-fed mice exhibit significantly lower fat mass than diet-matched wild-type controls
• however, lean body mass and food intake are relatively normal after HFD feeding
|
|
• adipocytes differentiated from E13.5 mutant MEFs show significantly smaller lipid droplets (LDs) than wild-type adipocytes, despite normal expression of adipogenic markers and lipogenic genes
• transduction of ApoL6-HA adenovirus into adipocytes differentiated from mutant MEFs restores lipid accumulation and lipid droplet size
|
|
• HFD-fed mice exhibit a significantly smaller white adipocyte size than diet-matched wild-type controls
|
 |
• after HFD feeding for 10 wks, epididymal WAT weight is significantly lower than in diet-matched wild-type controls
|
|
• after HFD feeding for 10 wks, perirenal WAT weight is significantly lower than in diet-matched wild-type controls
|
|
• HFD-fed mice exhibit significantly lower WAT mass than diet-matched wild-type controls
• however, BAT and liver weights are normal during HFD feeding
|
homeostasis/metabolism
|
• mice are protected from HFD-induced obesity with significantly lower body weight gain, white adipose tissue (WAT) mass and WAT depot weights, smaller white adipocyte size, and less crown-like structures and inflammation in WAT than diet-matched wild-type controls
|
|
• after HFD feeding, serum total cholesterol levels are significantly lower in the fed condition than in wild-type controls
|
|
• after HFD feeding, serum HDL cholesterol levels are significantly lower in the fed condition than in wild-type controls
|
|
• serum free fatty acid (FFA) levels are significantly higher in the fasted condition
• however, serum FFA levels are normal in the fed condition
|
|
• after HFD feeding, serum triacylglycerol (TAG) levels are significantly lower in the fed condition than in wild-type controls
|
|
• after HFD feeding for 10 wks, mice show significantly lower glucose levels at 15, 30 and 60 min after glucose injection than diet-matched wild-type controls
|
|
• after HFD feeding for 10 wks, mice show improved insulin sensitivity during ITT
|
|
• adipocytes differentiated from E13.5 mutant MEFs show significantly higher FFA release than wild-type adipocytes under both basal and isoproterenol-stimulated conditions
• dispersed adipocytes isolated from WAT show significantly higher FFA and glycerol release than wild-type adipocytes under both basal and stimulated conditions
• transduction of ApoL6-HA adenovirus into adipocytes differentiated from MEFs lowers FFA release to wild-type levels under both conditions
• treatment of dispersed adipocytes from WAT with Atglistatin (a PNPLA2/ATGL inhibitor), CAY10499 (an HSL inhibitor) or both lowers FFA release to a similar extent as in wild-type cells under both conditions
|
immune system
|
• HFD-fed mice show significantly less crown-like structures and lower mRNA levels of inflammatory markers in WAT and liver than diet-matched wild-type controls
|
cellular
|
• adipocytes differentiated from E13.5 mutant MEFs show significantly smaller lipid droplets (LDs) than wild-type adipocytes, despite normal expression of adipogenic markers and lipogenic genes
• transduction of ApoL6-HA adenovirus into adipocytes differentiated from mutant MEFs restores lipid accumulation and lipid droplet size
|
