Phenotypes associated with this allele

• Allele Symbol: Tg(Fabp4-Apol6)#Hsul
• Allele Name: transgene insertion, Hei Sul
• Allele ID: MGI:7578893
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Fabp4-Apol6)#Hsul/0	C57BL/6J-Tg(Fabp4-Apol6)#Hsul	MGI:7593628

Genotype
MGI:7593628

tg1

• Allelic Composition: Tg(Fabp4-Apol6)#Hsul/0
• Genetic Background: C57BL/6J-Tg(Fabp4-Apol6)#Hsul

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased body fat mass ( J:344362 )

• after 7 wks of high-fat diet (HFD) feeding, mice show a significantly higher fat mass than diet-matched wild-type controls

• however, lean body mass and food intake are relatively normal after HFD feeding

increased body weight ( J:344362 )

• at 11-12 wks of age, mice fed a normal chow diet (NCD) exhibit a significantly higher body weight than wild-type controls

• after 7 wks of HFD feeding, mice show a significantly higher body weight than diet-matched wild-type controls

adipose tissue

increased body fat mass ( J:344362 )

• after 7 wks of high-fat diet (HFD) feeding, mice show a significantly higher fat mass than diet-matched wild-type controls

• however, lean body mass and food intake are relatively normal after HFD feeding

increased white fat cell size ( J:344362 )

• at 24 wks of age, NCD-fed mice exhibit a significantly larger white adipocyte size than wild-type controls, with no histological differences noted in BAT

• after 12 wks of HFD feeding, mice show significantly larger white adipocytes than diet-matched controls

increased epididymal fat pad weight ( J:344362 )

♂ • at 24 wks of age, NCD-fed mice exhibit a significantly higher epididymal WAT weight than wild-type controls

♂ • after 12 wks of HFD feeding, mice show a significantly higher epididymal WAT weight than diet-matched wild-type controls

increased renal fat pad weight ( J:344362 )

• at 24 wks of age, NCD-fed mice exhibit a significantly higher perirenal WAT weight than wild-type controls

• after 12 wks of HFD feeding, mice show a significantly higher perirenal WAT weight than diet-matched wild-type controls

increased white adipose tissue mass ( J:344362 )

• at 11-12 wks of age, mice fed a NCD exhibit a significantly higher WAT mass than wild-type controls

• after 12 wks of HFD feeding, mice show a significantly higher WAT mass than diet-matched wild-type controls

• however, BAT, liver and other organ weights as well as expression of adipogenic transcription factors and lipogenic genes in WAT are normal on NCD

white adipose tissue inflammation ( J:344362 )

• HFD-fed mice exhibit significantly higher expression of inflammatory markers in WAT than diet-matched wild-type controls

homeostasis/metabolism

increased circulating cholesterol level ( J:344362 )

• serum total cholesterol levels are significantly higher in both the NCD- and HFD-fed condition than in diet-matched wild-type controls

increased circulating HDL cholesterol level ( J:344362 )

• serum HDL cholesterol levels are significantly higher in the NCD-fed condition and strongly trend towards higher levels in the HDF-fed condition

increased circulating LDL cholesterol level ( J:344362 )

• serum LDL cholesterol levels are significantly higher in both the NCD- and HFD-fed condition than in diet-matched wild-type controls

decreased circulating free fatty acids level ( J:344362 )

• serum FFA levels are significantly lower under both NCD-fed and fasted conditions

• however, after HFD feeding, serum FFA levels are normal in the fed condition

abnormal circulating glycerol level ( J:344362 )

• isoproterenol-treated mice show a significantly lower increase in glycerol release with serum glycerol levels only 4-fold higher than basal levels at 15 and 30 min post-injection versus 16- and 8-fold, respectively, in similarly treated wild-type controls

increased circulating triglyceride level ( J:344362 )

• serum triacylglycerol (TAG) levels are significantly higher in both the NCD- and HFD-fed condition than in diet-matched wild-type controls

impaired glucose tolerance ( J:344362 )

• HFD-fed mice are more glucose intolerant than diet-matched wild-type controls

• however, NCD-fed mice show normal glucose tolerance

insulin resistance ( J:344362 )

• HFD-fed mice are more insulin resistant than diet-matched wild-type controls

• however, NCD-fed mice show normal insulin tolerance

increased liver triglyceride level ( J:344362 )

• HFD-fed mice exhibit significantly higher liver TAG levels than diet-matched wild-type controls

impaired lipolysis ( J:344362 )

• after treatment with isoproterenol (to stimulate whole-body lipolysis), mice exhibit a significant decrease in lipolysis from adipose tissue, as determined by serum glycerol levels

• dispersed adipocytes isolated from WAT show significantly lower FFA and glycerol release than wild-type adipocytes under both basal and stimulated conditions

liver/biliary system

increased liver triglyceride level ( J:344362 )

• HFD-fed mice exhibit significantly higher liver TAG levels than diet-matched wild-type controls

increased susceptibility to diet-induced hepatic steatosis ( J:344362 )

• HFD-fed mice exhibit markedly higher lipid accumulation in the liver than diet-matched wild-type controls by Oil Red O staining

• however, liver histology is normal on NCD

immune system

white adipose tissue inflammation ( J:344362 )

• HFD-fed mice exhibit significantly higher expression of inflammatory markers in WAT than diet-matched wild-type controls