Phenotypes associated with this allele

• Allele Symbol: Nat10^em2Cya
• Allele Name: endonuclease-mediated mutation 2, Cyagen Biosciences
• Allele ID: MGI:7585238
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nat10^em2Cya/Nat10^em2Cya	C57BL/6J-Nat10^em2Cya/Cya	MGI:7788845

Genotype
MGI:7788845

cn1

• Allelic Composition: Nat10^em2Cya/Nat10^em2Cya
• Genetic Background: C57BL/6J-Nat10^em2Cya/Cya

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

increased mechanical nociceptive threshold ( J:345384 )

♂ • on day 7 after sciatic nerve chronic constriction injury (CCI), male mice pre-injected with AAV-Cre into unilateral lumbar (L)3/4 dorsal root ganglia (DRGs) show significantly lower ipsilateral paw-withdrawal frequencies in response to mechanical stimuli [low (0.07 g) and medium (0.4 g) force von Frey filaments] than control mice pre-injected with AAV-Gfp

increased thermal nociceptive threshold ( J:345384 )

♂ • on day 7 after sciatic nerve CCI surgery, male mice pre-injected with AAV-Cre into unilateral L3/4 DRGs show a significantly higher ipsilateral paw-withdrawal latency in response to heat stimuli than control mice pre-injected with AAV-Gfp

homeostasis/metabolism

abnormal protein level ( J:345384 )

♂ • on day 7 after CCI surgery, male mice pre-injected with AAV-Cre show a significantly lower abundance of N4-acetylcytidine (ac4C) protein in ipsilateral L3/4 DRGs than control mice pre-injected with AAV-Gfp

decreased susceptibility to injury ( J:345384 )

♂ • on day 7 after CCI surgery, male mice pre-injected with AAV-Cre into unilateral L3/4 DRGs show attenuated injury-induced mechanical and heat nociceptive hypersensitivities and significantly lower upregulation of p-ERK1/2 (a marker of neuronal hyperactivation) and GFAP (a marker of astrocytic hyperactivation) in the ipsilateral dorsal spinal horn than control mice pre-injected with AAV-Gfp

♂ • however, no defects in locomotor function (placing, grasping, and righting reflexes) are observed after CCI surgery in response to AAV-Cre or AAV-Gfp treatment