Phenotypes associated with this allele

• Allele Symbol: Wac^em2Cya
• Allele Name: endonuclease-mediated mutation 2, Cyagen Biosciences
• Allele ID: MGI:7587544
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd19^tm1(cre)Cgn/Cd19^+ Wac^em2Cya/Wac^em2Cya	B6.Cg-Cd19^tm1(cre)Cgn Wac^em2Cya	MGI:7783621

Genotype
MGI:7783621

cn1

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Wac^em2Cya/Wac^em2Cya
• Genetic Background: B6.Cg-Cd19^tm1(cre)Cgn Wac^em2Cya

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased B cell proliferation ( J:345327 )

• under in vitro plasma cell (PC) differentiation conditions, proliferation of CFSE-labeled B cells is halted at division 2-4 upon 5 and 20 ug/mL LPS stimulation

• after LPS stimulation, splenic B cells show significantly reduced BrdU incorporation relative to wild-type cells

abnormal plasma cell differentiation ( J:345327 )

• 3 days after stimulation with LPS, isolated splenic B cells show a 50% reduction of in vitro PC differentiation

• however, LPS-induced B cell activation is unaffected, as indicated by normal upregulation of various activation markers

• moreover, expression of master transcription factors essential for PC differentiation is largely normal

decreased plasma cell number ( J:345327 )

• significant decrease in NP-specific IgG1 antibody-secreting plasma cells in the spleen and bone marrow on day 10 after NP-CGG immunization

• significant decrease in the frequencies of NP-specific IgM and IgG3 antibody-secreting plasma cells in the spleen on day 5 after NP-LPS immunization

• however, the frequencies and numbers of B220+Fas+CD38- germinal center (GC) B cells in spleen are normal on day 10 after NP-CGG immunization

decreased IgG1 level ( J:345327 )

• significant decrease in serum levels of NP-specific IgG1 at all time points after NP-CGG immunization

decreased IgG2b level ( J:345327 )

• significant decrease in serum levels of NP-specific IgG2b on days 7, 14 and 21 after NP-CGG immunization

decreased IgG3 level ( J:345327 )

• significant decrease in serum levels of NP-specific IgG3 on days 7, 14 and 21 after NP-LPS immunization

• significant decrease in serum levels of NP-specific IgG3 at all time points after NP-CGG immunization

decreased IgM level ( J:345327 )

• slight but significant decrease in serum levels of NP-specific IgM on days 21 and 28, but not on days 7 14, after NP-CGG immunization

• significant decrease in serum levels of NP-specific IgM on days 7, 14 and 21 after NP-LPS immunization

impaired humoral immune response ( J:345327 )

• severely compromised T cell-dependent and T cell-independent antibody responses

hematopoietic system

hematopoietic system phenotype ( J:345327 )

N • 6- to 14-week-old mice show normal B cell development in the bone marrow and no significant changes in peripheral B cell populations in spleen relative to wild-type controls

decreased B cell proliferation ( J:345327 )

• under in vitro plasma cell (PC) differentiation conditions, proliferation of CFSE-labeled B cells is halted at division 2-4 upon 5 and 20 ug/mL LPS stimulation

• after LPS stimulation, splenic B cells show significantly reduced BrdU incorporation relative to wild-type cells

abnormal plasma cell differentiation ( J:345327 )

• 3 days after stimulation with LPS, isolated splenic B cells show a 50% reduction of in vitro PC differentiation

• however, LPS-induced B cell activation is unaffected, as indicated by normal upregulation of various activation markers

• moreover, expression of master transcription factors essential for PC differentiation is largely normal

decreased plasma cell number ( J:345327 )

• significant decrease in NP-specific IgG1 antibody-secreting plasma cells in the spleen and bone marrow on day 10 after NP-CGG immunization

• significant decrease in the frequencies of NP-specific IgM and IgG3 antibody-secreting plasma cells in the spleen on day 5 after NP-LPS immunization

• however, the frequencies and numbers of B220+Fas+CD38- germinal center (GC) B cells in spleen are normal on day 10 after NP-CGG immunization

decreased IgG1 level ( J:345327 )

• significant decrease in serum levels of NP-specific IgG1 at all time points after NP-CGG immunization

decreased IgG2b level ( J:345327 )

• significant decrease in serum levels of NP-specific IgG2b on days 7, 14 and 21 after NP-CGG immunization

decreased IgG3 level ( J:345327 )

• significant decrease in serum levels of NP-specific IgG3 at all time points after NP-CGG immunization

• significant decrease in serum levels of NP-specific IgG3 on days 7, 14 and 21 after NP-LPS immunization

decreased IgM level ( J:345327 )

• slight but significant decrease in serum levels of NP-specific IgM on days 21 and 28, but not on days 7 14, after NP-CGG immunization

• significant decrease in serum levels of NP-specific IgM on days 7, 14 and 21 after NP-LPS immunization

cellular

decreased B cell proliferation ( J:345327 )

• under in vitro plasma cell (PC) differentiation conditions, proliferation of CFSE-labeled B cells is halted at division 2-4 upon 5 and 20 ug/mL LPS stimulation

• after LPS stimulation, splenic B cells show significantly reduced BrdU incorporation relative to wild-type cells

abnormal epigenetic regulation of gene expression ( J:345327 )

• significant decrease in histone H2B ubiquitination (ubH2B) levels in resting B cells, LPS-stimulated B220+CD138- B cells, and B220^lo CD138+ LPS-induced plasma cells

• reduction in ubH2B is correlated with downregulated expression of several metabolism-related genes in LPS-stimulated B cells