Phenotypes associated with this allele

• Allele Symbol: Tubb4b^{tm2b(EUCOMM)Wtsi}
• Allele Name: targeted mutation 2b, Wellcome Trust Sanger Institute
• Allele ID: MGI:7593955
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tubb4b^tm2b(EUCOMM)Wtsi/Tubb4b^tm2b(EUCOMM)Wtsi	involves: C57BL/6 * FVB	MGI:7593957
cx2	Tubb4a^tm1(KOMP)Wtsi/Tubb4a^tm1(KOMP)Wtsi Tubb4b^tm2b(EUCOMM)Wtsi/Tubb4b^tm2b(EUCOMM)Wtsi	involves: C57BL/6 * FVB	MGI:7593963

Genotype
MGI:7593957

hm1

• Allelic Composition: Tubb4b^{tm2b(EUCOMM)Wtsi}/Tubb4b^{tm2b(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6 * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:344732 )

• proportion of homozygous mice are reduced to 13% and 11% at P4-P6 and P7-P10, respectively

• over 45% die before P13 and over 88% die before P21

growth/size/body

postnatal growth retardation ( J:344732 )

• appear smaller after P3

nervous system

hydrocephaly ( J:344732 )

• seen in over 80% of pups surviving past P21

cellular

abnormal oviduct epithelium motile cilium morphology ( J:344732 )

• severe truncation of cilia on multi-ciliated cells

• however, multi-cilia on ependymal cells are not truncated

abnormal respiratory motile cilium morphology ( J:344732 )

• severe truncation of cilia on multi-ciliated cells

• multi-cilia show impaired extension of the ciliary axoneme or have deformed axonemes

respiratory system

abnormal respiratory motile cilium morphology ( J:344732 )

• severe truncation of cilia on multi-ciliated cells

• multi-cilia show impaired extension of the ciliary axoneme or have deformed axonemes

abnormal branching involved in lung morphogenesis ( J:344732 )

• show less fine tissue arborization in small airways in the lungs at P10-P12 resulting in an increase in non-cellular space

vision/eye

vision/eye phenotype ( J:344732 )

N • no defects are detected in the retina

reproductive system

abnormal oviduct epithelium motile cilium morphology ( J:344732 )

• severe truncation of cilia on multi-ciliated cells

• however, multi-cilia on ependymal cells are not truncated

Genotype
MGI:7593963

cx2

• Allelic Composition: Tubb4a^{tm1(KOMP)Wtsi}/Tubb4a^{tm1(KOMP)Wtsi}; Tubb4b^{tm2b(EUCOMM)Wtsi}/Tubb4b^{tm2b(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6 * FVB

cellular

abnormal respiratory motile cilium morphology ( J:344732 )

• severe truncation of cilia on multi-ciliated cells although occasional longer cilia are seen

• no cilia display the symmetric 9 microtuble doublet axonemes, all show the presence of singlets

respiratory system

abnormal respiratory motile cilium morphology ( J:344732 )

• severe truncation of cilia on multi-ciliated cells although occasional longer cilia are seen

• no cilia display the symmetric 9 microtuble doublet axonemes, all show the presence of singlets