All Phenotypes Cfap119<em1Gaof> MGI Mouse

oligozoospermia ( J:345163 )

• sperm count is dramatically decreased

teratozoospermia ( J:345163 )

• most sperm are malformed

abnormal sperm flagellum morphology ( J:345163 )

• immunofluorescence of the sperm flagella marker AQP3 (aquaporin 3) showed only a punctate AQP3 signal in testes

• most sperm show short, coiled or helical flagella

abnormal sperm axoneme morphology ( J:345163 )

• percentage of multiple sperm axonemal structural defects is significantly increased

abnormal sperm mitochondrial morphology ( J:345163 )

• percentage of abnormal sperm mitochondria significantly increased

abnormal sperm fibrous sheath morphology ( J:345163 )

• SEM revealed sperm with a coiled fibrous sheath

coiled sperm flagellum ( J:345163 )

short sperm flagellum ( J:345163 )

abnormal sperm head morphology ( J:345163 )

• percentage of abnormal sperm heads is significantly increased

abnormal acrosome morphology ( J:345163 )

• PNA-positive acrosomes appear abnormal

elongated manchette ( J:345163 )

• elongating spermatids exhibit abnormally elongated manchettes at steps 11-12

asthenozoospermia ( J:345163 )

• percentage of motile spermatozoa is dramatically reduced

small testis ( J:345163 )

• adult testis size is significantly reduced

decreased testis weight ( J:345163 )

• adult testis/body weight ratio is significantly reduced

• however, adult body weight is normal

abnormal spermiogenesis ( J:345163 )

• only a small number of mature spermatozoa are noted in the epididymis

• however, no obvious histological changes are detected in the seminiferous tubules and early stage of germ cell development is normal

abnormal manchette assembly ( J:345163 )

male infertility ( J:345163 )

• males are completely sterile

• however, no obvious developmental defects are observed in adult mice

cellular phenotype ( J:345163 )

N	• tracheal epithelial cells show a normal number of ciliated cells and normal cilial morphology • mouse embryonic fibroblasts (MEFs) show normal serum starvation-induced ciliogenesis with normal primary cilia number and cilial length