All Phenotypes Creb3<Gt1Mbac> MGI Mouse

abnormal body composition ( J:345514 )

• after HFD feeding for 8 weeks, lean muscle percentage is significantly increased by 15.5% in females and modestly increased by 4.3% in males relative to HFD-fed wild-type controls

decreased body fat mass ( J:345514 )

• both females and males fed a HFD for 8 weeks show a drastic reduction in absolute fat tissue mass relative to HFD-fed wild-type controls

decreased lean body mass ( J:345514 )

• despite an increase in lean mass percentage, both females and males fed a HFD for 8 weeks show a significant reduction in absolute lean tissue mass relative to HFD-fed wild-type controls

decreased susceptibility to diet-induced obesity ( J:345514 )

• when 8-week-old mice are fed a HFD for 8 weeks, both males and females show reduced body fat; HFD weight gain is reduced by 34% in males and by 39.5% in females relative to HFD-fed controls

• however, average HFD food intake and total fecal lipids are relatively normal in both sexes

homeostasis/metabolism phenotype ( J:345514 )

N	• whether fed a control diet (CD) or a high-fat diet (HFD) for 8 weeks, both male and female mice show normal plasma levels of total cholesterol, HDL cholesterol, non-esterified fatty acids (NEFA), and triglycerides (TG) relative to diet-matched wild-type controls

decreased fasting circulating glucose level ( J:345514 )

• after HFD feeding, both female and male mice exhibit significantly lower fasted blood glucose levels than HFD-fed wild-type controls

• however, no differences in fasted blood glucose levels are noted between HFD-fed and CD-fed mice

decreased circulating insulin level ( J:345514 )

• CD-fed males show a mild reduction in fasted blood insulin levels relative to CD-fed wild-type males

• however, fasted blood insulin levels are relatively normal in CD-fed females

decreased circulating leptin level ( J:345514 )

• CD-fed females show a severe 3.26-fold reduction in fasted blood leptin levels relative to CD-fed wild-type females

• however, fasted blood leptin levels are relatively normal in CD-fed males

increased energy expenditure ( J:345514 )

• HFD-fed female and male mice exhibit increased energy expenditure relative to HFD-fed wild-type controls

• however, no differences in HFD energy intake are observed

decreased susceptibility to diet-induced obesity ( J:345514 )

• when 8-week-old mice are fed a HFD for 8 weeks, both males and females show reduced body fat; HFD weight gain is reduced by 34% in males and by 39.5% in females relative to HFD-fed controls

• however, average HFD food intake and total fecal lipids are relatively normal in both sexes

increased respiratory quotient ( J:345514 )

• after HFD feeding, respiratory exchange ratios (RERs) are increased 17.5% and 25% in males and females, respectively, relative to HFD-fed wild-type controls

• however, no differences in RERs are noted between any of the CD-fed groups

improved glucose tolerance ( J:345514 )

• HFD-fed males show improved glucose tolerance in an IGTT test, with a reduced area-under-curve (AUC) after an overnight fast relative to HFD-fed wild-type males

• however, HFD-fed females show little to no reduction in AUC

abnormal lipid level ( J:345514 )

• HFD-fed males show drastically reduced hepatic glycerolipid levels, with a 71% decrease in hepatic TG, a 55% decrease in monoacylglycerol (MAG), and a 68% decrease in diacylglycerol (DAG) relative to HFD-fed wild-type males

abnormal phospholipid level ( J:345514 )

• HFD-fed males show a significant increase in the hepatic levels of membrane phospholipids; liver phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS) are increased by 60%, 78%, and 52%, respectively

decreased liver triglyceride level ( J:345514 )

• HFD-fed males have a total liver TG content that is 1.7-fold lower than that in HFD-fed wild-type males

• however, no differences in total liver TG are noted between HFD-fed female groups, or in any of the CD-fed groups

decreased skeletal muscle triglyceride level ( J:345514 )

• HFD-fed females show a 3.6-fold reduction of total TG content in skeletal muscle relative to HFD-fed wild-type females

• however, no differences in total muscle TG are found between male groups and diets

increased basal metabolism ( J:345514 )

• after HFD feeding, body heat production is increased 46% and 40% in males and females, respectively, relative to HFD-fed wild-type controls

• however, no differences in body heat are noted between the CD-fed groups

decreased body fat mass ( J:345514 )

• both females and males fed a HFD for 8 weeks show a drastic reduction in absolute fat tissue mass relative to HFD-fed wild-type controls

increased white fat cell number ( J:345514 )

• on both CD and HFD, both male and female mice exhibit a significantly greater number of smaller adipocytes in gonadal/epidydimal adipose tissue than diet-matched wild-type controls

decreased white fat cell size ( J:345514 )

• on both CD and HFD, both male and female mice exhibit a significantly greater number of smaller adipocytes in gonadal/epidydimal adipose tissue than diet-matched wild-type controls

decreased percent body fat/body weight ( J:345514 )

• females fed a HFD for 8 weeks show a significantly lower percent body fat (26.07%) than HFD-fed wild-type females (41.17%)

• however, HFD-fed males show no differences in % body fat relative to HFD-fed wild-type males