Phenotypes associated with this allele

• Allele Symbol: Trim29^{tm1c(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:7608151
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+ Kras^tm4Tyj/Kras^+ Tg(Pdx1-cre)89.1Dam/0 Trim29^tm1c(EUCOMM)Wtsi/Trim29^tm1c(EUCOMM)Wtsi Trp53^tm1Brn/Trp53^+	involves: 129 * C57BL/6 * CBA	MGI:7736726
cn2	Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+ Tg(Pdx1-cre)89.1Dam/0 Trim29^tm1c(EUCOMM)Wtsi/Trim29^tm1c(EUCOMM)Wtsi	involves: 129 * C57BL/6 * CBA	MGI:7736742
cn3	Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+ Kras^tm4Tyj/Kras^+ Tg(Pdx1-cre)89.1Dam/0 Trim29^tm1c(EUCOMM)Wtsi/Trim29^tm1c(EUCOMM)Wtsi	involves: 129 * C57BL/6 * CBA	MGI:7736747
cn4	Trim29^tm1c(EUCOMM)Wtsi/Trim29^tm1c(EUCOMM)Wtsi Tg(Myh6-cre)2182Mds/0	involves: C57BL/6J * C57BL/6N * C57BL/6NTac	MGI:7662823
cn5	Trim29^tm1c(EUCOMM)Wtsi/Trim29^tm1c(EUCOMM)Wtsi Tg(Pdx1-cre)6Tuv/0	involves: C57BL/6N * FVB/N	MGI:7736562

Genotype
MGI:7736726

cn1

• Allelic Composition: Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺; Kras^tm4Tyj/Kras⁺; Tg(Pdx1-cre)89.1Dam/0; Trim29^{tm1c(EUCOMM)Wtsi}/Trim29^{tm1c(EUCOMM)Wtsi}; Trp53^tm1Brn/Trp53⁺
• Genetic Background: involves: 129 * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:289183 )

N • 73.3% of 3-month old and 75% of 12-month old mice show normal pancreas

N • although 26.7% of 3-month old and 25% of 12-month old mice show rare acinar-ductal metaplasia and low-grade pancreatic intraepithelial neoplasia (PanIN1) lesions, these lesions express TRIM29 due to incomplete recombination of the floxed allele

mortality/aging

mortality/aging ( J:289183 )

N • all mice are alive at 12 months

Genotype
MGI:7736742

cn2

• Allelic Composition: Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺; Tg(Pdx1-cre)89.1Dam/0; Trim29^{tm1c(EUCOMM)Wtsi}/Trim29^{tm1c(EUCOMM)Wtsi}
• Genetic Background: involves: 129 * C57BL/6 * CBA

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:289183 )

N • mice with cerulein-induced acute pancreatitis show reversion of acinar-ductal metaplasia lesions to normal acinar morphology after 7 days of recovery similarly as in wild-type mice

Genotype
MGI:7736747

cn3

• Allelic Composition: Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺; Kras^tm4Tyj/Kras⁺; Tg(Pdx1-cre)89.1Dam/0; Trim29^{tm1c(EUCOMM)Wtsi}/Trim29^{tm1c(EUCOMM)Wtsi}
• Genetic Background: involves: 129 * C57BL/6 * CBA

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:289183 )

N • mice with cerulein-induced acute pancreatitis show reversion of acinar-ductal metaplasia lesions to normal acinar morphology after 7 days of recovery as in wild-type mice

decreased gland tumor incidence ( J:289183 )

• primary pancreatic acinar cells from 1.5-month-old mice grown in 3D collagen culture show attenuated KRAS^tm4Tyj-induced acinar cell transdifferentiation and acinar-ductal metaplasia formation

neoplasm

decreased gland tumor incidence ( J:289183 )

• primary pancreatic acinar cells from 1.5-month-old mice grown in 3D collagen culture show attenuated KRAS^tm4Tyj-induced acinar cell transdifferentiation and acinar-ductal metaplasia formation

Genotype
MGI:7662823

cn4

• Allelic Composition: Trim29^{tm1c(EUCOMM)Wtsi}/Trim29^{tm1c(EUCOMM)Wtsi}; Tg(Myh6-cre)2182Mds/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * C57BL/6NTac
• Cell Lines: EPD0439_4_D01

mortality/aging

decreased susceptibility to Picornaviridae infection induced morbidity/mortality ( J:349246 )

♂ • following i.p. infection with Coxsackievirus B3 (CVB3, strain Nancy), mice show significantly improved survival rates relative to CVB3-infected controls

immune system

increased T cell proliferation ( J:349246 )

♂ • when myeloid-derived suppressor cells (MDSCs) from 3-day CVB3-infected hearts are cocultured with negatively selected CD3+ T cells and stimulated with anti-CD3 and anti-CD28 antibodies, the % of proliferating CD8+ T cells is significantly higher than that in controls

increased interferon level ( J:349246 )

♂ • following CVB3 infection, mice show significantly higher IFN-alpha and IFN-beta levels in the heart than CVB3-infected controls

decreased interleukin level ( J:349246 )

♂ • following CVB3 infection, mice show significantly lower IL-6 and IL-1beta levels in the heart than CVB3-infected controls

abnormal tumor necrosis factor level ( J:349246 )

♂ • following CVB3 infection, mice show significantly lower TNF levels in the heart than CVB3-infected controls

increased interferon-gamma secretion ( J:349246 )

♂ • upon in vitro stimulation with PMA/ionomycin, CD8+ T cells show a significantly enhanced IFN-gamma producing ability in the heart and spleen from 2-day CVB3-infected mice

♂ • however, the IFN-gamma-producing ability of CD8+ T cells in spleen is similar to that in untreated controls before CVB3 infection

decreased inflammatory response ( J:349246 )

♂ • 2 days after CVB3 infection, mice show significantly lower cell numbers of monocytic MDSCs (CD11b+Ly6C+Ly6G-), macrophages, neutrophils, B cells and T cells infiltrated in the heart than CVB3-infected controls

decreased susceptibility to Picornaviridae infection ( J:349246 )

♂ • following i.p. infection with Coxsackievirus B3 (CVB3, strain Nancy), mice show significantly less inflammation and infiltration of inflammatory cells in heart, lower heart weight/baseline body weight, improved cardiac function (as assessed by ejection fraction and fractional shortening), reduced viral titers in heart, pancreas and spleen homogenates, and lower levels of cardiac inflammatory cytokines (IL-6, IL-1beta, and TNF) than CVB3-infected controls, indicating protection from viral myocarditis

decreased susceptibility to Picornaviridae infection induced morbidity/mortality ( J:349246 )

♂ • following i.p. infection with Coxsackievirus B3 (CVB3, strain Nancy), mice show significantly improved survival rates relative to CVB3-infected controls

cellular

decreased fetal cardiomyocyte apoptosis ( J:349246 )

♂ • in vitro, primary neonatal cardiomyocytes infected with CVB3 show dramatically reduced expression of CHOP, cleaved caspase-3, and BAX, indicating reduced EIF2AK3-mediated ER stress and apoptosis

increased T cell proliferation ( J:349246 )

♂ • when myeloid-derived suppressor cells (MDSCs) from 3-day CVB3-infected hearts are cocultured with negatively selected CD3+ T cells and stimulated with anti-CD3 and anti-CD28 antibodies, the % of proliferating CD8+ T cells is significantly higher than that in controls

decreased endoplasmic reticulum stress ( J:349246 )

♂ • following infection with CVB3, primary neonatal cardiomyocytes from 2-day-old mice show dramatically reduced phosphorylation of EIF2AK3 (eukaryotic translation initiation factor 2 alpha kinase 3, also known as PERK), suggesting reduced EIF2AK3-mediated ER stress

homeostasis/metabolism

decreased circulating creatine kinase level ( J:349246 )

♂ • following CVB3 infection, mice show dramatically reduced circulating creatine kinase levels relative to CVB3-infected controls

increased interferon level ( J:349246 )

♂ • following CVB3 infection, mice show significantly higher IFN-alpha and IFN-beta levels in the heart than CVB3-infected controls

decreased interleukin level ( J:349246 )

♂ • following CVB3 infection, mice show significantly lower IL-6 and IL-1beta levels in the heart than CVB3-infected controls

abnormal tumor necrosis factor level ( J:349246 )

♂ • following CVB3 infection, mice show significantly lower TNF levels in the heart than CVB3-infected controls

hematopoietic system

increased T cell proliferation ( J:349246 )

♂ • when myeloid-derived suppressor cells (MDSCs) from 3-day CVB3-infected hearts are cocultured with negatively selected CD3+ T cells and stimulated with anti-CD3 and anti-CD28 antibodies, the % of proliferating CD8+ T cells is significantly higher than that in controls

decreased myeloid cell number ( J:349246 )

♂ • 2 days after CVB3 infection, mice show a significantly lower frequency of myeloid-derived suppressor cells (CD11b+ Gr1+ MDSCs) in the heart and spleen than CVB3-infected controls

♂ • however, the frequency of MDSCs in spleen is similar to that in untreated controls before CVB3 infection

cardiovascular system

decreased fetal cardiomyocyte apoptosis ( J:349246 )

♂ • in vitro, primary neonatal cardiomyocytes infected with CVB3 show dramatically reduced expression of CHOP, cleaved caspase-3, and BAX, indicating reduced EIF2AK3-mediated ER stress and apoptosis

muscle

decreased fetal cardiomyocyte apoptosis ( J:349246 )

♂ • in vitro, primary neonatal cardiomyocytes infected with CVB3 show dramatically reduced expression of CHOP, cleaved caspase-3, and BAX, indicating reduced EIF2AK3-mediated ER stress and apoptosis

Genotype
MGI:7736562

cn5

• Allelic Composition: Trim29^{tm1c(EUCOMM)Wtsi}/Trim29^{tm1c(EUCOMM)Wtsi}; Tg(Pdx1-cre)6Tuv/0
• Genetic Background: involves: C57BL/6N * FVB/N

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:289183 )

N • mice show normal pancreatic weight, morphology, and histology up to 12 months of age

mortality/aging

mortality/aging ( J:289183 )

N • mice are born at the expected frequency