Analysis Tools
mortality/aging
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• fewer than expected embryos at E17.5 (18% vs 25%)
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• additional homozygous mice die before weaning with 72% of the expected homozygous mice not reaching adulthood
• litter size did not impact the number of homozygous mice surviving
• 81% of homozygous mice born alive die within the first 3 postnatal days compared to 9% of wild-type mice
• mice that survive past the first few days are fertile and do not display any gross abnormalities
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respiratory system
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• impaired lung maturation at E17.5 with lungs still at the canalicular stage when lungs in wild-type embryos have reached the saccular stage
• number and size of small airways and distal airspaces are significantly smaller atE17.5
• developmental delay correlates to overall embryo size
• formation and maturation of distal epithelial cells are slightly behind schedule at E17.5
• delay in lung development is detectable at E12.5
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• lung explants from E12.5 embryos show significantly reduced numbers of peripheral branching buds
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• at E17.5
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• smaller at E17.5 but still able to synthesize surfactant protein C
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• at E17.5
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small lung
(
J:345100
)
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• at E17.5
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embryo
| N |
• despite expression in the placenta no histological abnormalities are detected in the placentas
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homeostasis/metabolism
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• at E17.5, alkaline phosphatase activity is significantly reduced
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• at E17.5 in amniotic fluid
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growth/size/body
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• at E17.5
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• smaller at E11.5, E14.5, and E17.5
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