Analysis Tools
behavior/neurological
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• mutant females are more active than mutant males
• mutant females are more active than wild-type mice during the dark, but not the light, cycle
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cardiovascular system
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• surge blood pressure (the delta between the lowest blood pressure during the light cycle and the highest blood pressure) is lower in females compared to mutant males for diastolic blood pressure in contrast to wild-type mice which show no difference in surge blood pressure between the sexes
• however, no differences in pulse pressure are seen in males or females
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• males exhibit a small decrease in diastolic blood pressure, which is significant during the light cycle, without a change in systolic blood pressure
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• females exhibit an increase in mean arterial pressure and diastolic blood pressure in both light and dark cycles compared to wild-type females
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• pulse wave velocity is increased in 10-week-old female aortas indicating increased vascular stiffness
• however, tensile properties are similar to wild-type in both intact and decellularized aortic rings of 10-week-old females and no differences in the wall thickness and diameter or aortic rings is seen
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• ex vivo vascular reactivity studies show that aortic rings from males exhibit less constriction to phenylephrine, but no differences in constriction to potassium chloride or relaxation to acetylcholine
• however, mesenteric arteries show similar constriction to potassium chloride and phenylephrine
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• ex vivo vascular reactivity studies show that aortic rings from females exhibit higher maximal contractility induced by potassium chloride compared to wild-type
• however, similar responses to phenylephrine, acetylcholine, and sodium nitroprusside are seen suggesting no impairments in vascular smooth muscle or endothelial function in females
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• mesentery arteries from males, but not females, exhibit increased relaxation to sodium nitroprusside
• however, mesenteric arteries show similar relaxation to acetylcholine as in controls
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muscle
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• pulse wave velocity is increased in 10-week-old female aortas indicating increased vascular stiffness
• however, tensile properties are similar to wild-type in both intact and decellularized aortic rings of 10-week-old females and no differences in the wall thickness and diameter or aortic rings is seen
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• ex vivo vascular reactivity studies show that aortic rings from males exhibit less constriction to phenylephrine, but no differences in constriction to potassium chloride or relaxation to acetylcholine
• however, mesenteric arteries show similar constriction to potassium chloride and phenylephrine
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• ex vivo vascular reactivity studies show that aortic rings from females exhibit higher maximal contractility induced by potassium chloride compared to wild-type
• however, similar responses to phenylephrine, acetylcholine, and sodium nitroprusside are seen suggesting no impairments in vascular smooth muscle or endothelial function in females
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• mesentery arteries from males, but not females, exhibit increased relaxation to sodium nitroprusside
• however, mesenteric arteries show similar relaxation to acetylcholine as in controls
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digestive/alimentary system
| N |
• gut microbiome is similar to that of wild-type mice
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growth/size/body
| N |
• mice exhibit no differences in body weight, kidney weight/body weight ratio, or heart weight/body weight ratio
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hematopoietic system
| N |
• mice exhibit no differences in hemoglobin
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homeostasis/metabolism
| N |
• mice exhibit no differences in blood electrolytes, glucose, blood urea nitrogen, or creatinine
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• males, but not females, show a decrease in renin mRNA, a reduction in the percentage of renin-positive to total glomeruli, and decreased plasma renin activity on two different diets (0.2% NaCl and 0.49% NaCl)
• males show a decrease in renin+ cells in the kidneys
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renal/urinary system
| N |
• mice exhibit no differences in glomerular filtration rate
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