Phenotypes associated with this allele

• Allele Symbol: Gnb1^em1Dgold
• Allele Name: endonuclease-mediated mutation 1, David B Goldstein
• Allele ID: MGI:7620469
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gnb1^em1Dgold/Gnb1^em1Dgold	C57BL/6NJ-Gnb1^em1Dgold	MGI:7621196
ht2	Gnb1^em1Dgold/Gnb1^+	C57BL/6NJ-Gnb1^em1Dgold	MGI:7621197
ht3	Gnb1^em1Dgold/Gnb1^+	involves: 129P2/OlaHsd * C57BL/6NJ * FVB	MGI:7621199

Genotype
MGI:7621196

hm1

• Allelic Composition: Gnb1^em1Dgold/Gnb1^em1Dgold
• Genetic Background: C57BL/6NJ-Gnb1^em1Dgold

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:346983 )

• no mice born

Genotype
MGI:7621197

ht2

• Allelic Composition: Gnb1^em1Dgold/Gnb1⁺
• Genetic Background: C57BL/6NJ-Gnb1^em1Dgold

growth/size/body

decreased body size ( J:346983 )

• between ages P0 and 6 weeks

decreased body weight ( J:346983 )

• between ages P0-P6

behavior/neurological

behavior/neurological phenotype ( J:346983 )

N • normal grip strength between ages P4-P10

N • normal rearing behavior and time spent in center in open field test

N • normal behavior in rotarod test

N • normal behavior in contextual and cued fear conditioning tests

N • normal swimming speed in Morris water maze test

impaired spatial learning ( J:346983 )

• longer acquisition latency to platform in Morris water maze test

abnormal long-term spatial reference memory ( J:346983 )

• longer reversal latency to platform in Morris water maze test

impaired righting response ( J:346983 )

• faster at age P4 but slower at age P8-P10

abnormal locomotor behavior ( J:346983 )

• longer latency to turn 90 or 180 degrees from downward-facing start position on a slope between ages P4-P10

decreased locomotor activity ( J:346983 )

• shorter distance traveled in open field test

abnormal maternal behavior ( J:346983 )

♀ • lower than expected Mendelian ratio at age P0 owing to deficient maternal behavior

decreased vocalization ( J:346983 )

• fewer separation-induced ultrasonic vocalizations (USVs) between ages P5-P9

minimal clonic seizures ( J:346983 )

• significantly lower electroconvulsive threshold (ECT)

abnormal spike wave discharge ( J:346983 )

• highly frequent generalized bilateral spike-and- wave discharges (SWDs)

nervous system

minimal clonic seizures ( J:346983 )

• significantly lower electroconvulsive threshold (ECT)

abnormal spike wave discharge ( J:346983 )

• highly frequent generalized bilateral spike-and- wave discharges (SWDs)

Genotype
MGI:7621199

ht3

• Allelic Composition: Gnb1^em1Dgold/Gnb1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6NJ * FVB

behavior/neurological

behavior/neurological phenotype ( J:346983 )

N • normal grip strength between ages P4-P10

impaired righting response ( J:346983 )

• faster at age P4 but slower at ages P8-P10

abnormal locomotor behavior ( J:346983 )

• longer latency to turn 90 or 180 degrees from downward-facing start position on a slope between ages P4-P10

decreased vocalization ( J:346983 )

• fewer separation-induced ultrasonic vocalizations (USVs) between ages P4-P8

abnormal spike wave discharge ( J:346983 )

• highly frequent generalized bilateral spike-and- wave discharges (SWDs)

nervous system

abnormal spike wave discharge ( J:346983 )

• highly frequent generalized bilateral spike-and- wave discharges (SWDs)

growth/size/body

decreased body size ( J:346983 )

• between ages P0 and 6 weeks

decreased body weight ( J:346983 )

• between ages P4-P6

embryo

embryo phenotype ( J:346983 )

N • mice born at expected Mendelian ratio