All Phenotypes Ccdc183<em1Osb> MGI Mouse

oligozoospermia ( J:342837 )

• adult male mice have fewer spermatozoa; normal spermatozoa are difficult to observe in the cauda epididymis

teratozoospermia ( J:342837 )

• proportion of cauda epididymal spermatozoa with normal morphology is dramatically reduced

abnormal sperm flagellum morphology ( J:342837 )

• sperm tails are hardly found in testicular sections of adult males at all stages examined

• most spermatid flagella are located around round spermatids and only a few are found in the lumen of seminiferous tubules

abnormal outer dense fiber morphology ( J:342837 )

• cauda epididymal spermatozoa show absence of outer dense fiber structures in the principal piece

• outer dense fibers structures are abnormal in step 8 spermatids; outer dense fibers are not properly localized around the axoneme in step 16 spermatids

• most ODF2 is ectopically localized in step 10 spermatids; however, some ODF2 localizes normally

abnormal sperm axoneme morphology ( J:342837 )

• step 16 spermatids show collapsed axoneme structures and disintegrated microtubules during spermiogenesis

• cauda epididymal spermatozoa lack normal axonemes; fragmented microtubules attached to outer dense fibers are observed

• however, the 9 + 2 arrangement of microtubule doublets appears normal in step 6 spermatids

thick sperm flagellum ( J:342837 )

abnormal sperm mitochondrial sheath morphology ( J:342837 )

• mitochondrial sheaths without normal axoneme structure are observed in step 16 spermatids

abnormal sperm principal piece morphology ( J:342837 )

• nearly all principal pieces of cauda epididymal spermatozoa do not have fibrous sheath and outer dense fibers, although a faint axoneme is observed

abnormal sperm fibrous sheath morphology ( J:342837 )

• expression signals of the fibrous sheath-associated proteins AKAP3 and AKAP4 are dramatically reduced

• in elongated spermatids, AKAP3 is abnormally detected as dots without filament-like signals, suggesting impaired elongation of the fibrous sheath

• AKAP3 is scattered in the cytoplasm rather than found on the inner side of the fibrous sheath surface as in control spermatids

absent sperm fibrous sheath ( J:342837 )

• cauda epididymal spermatozoa show absence of a fibrous sheath in the principal piece

• a fibrous sheath surrounding axonemal microtubules and outer dense fibers is rarely observed; however, these structures are not compartmentalized by a flagellar membrane

abnormal sperm head morphology ( J:342837 )

• elongating spermatids exhibit abnormal head shapes due to abnormal sculpting by the manchette

abnormal manchette morphology ( J:342837 )

• step 12 spermatids exhibit abnormalities in manchette morphology and localization

• however, manchette microtubule structures appear normal

decreased sperm progressive motility ( J:342837 )

• progressive sperm rate is dramatically reduced

immotile sperm ( J:342837 )

• CASA revealed that spermatozoa are incapable of sperm motility and progression

abnormal seminiferous tubule morphology ( J:342837 )

• stage VIII seminiferous tubules exhibit a very low lumen volume and sperm tails are hardly detectable

small testis ( J:342837 )

• testes are grossly smaller than those in wild-type males

decreased testis weight ( J:342837 )

• average testicular weight is significantly lower than that in wild-type males

abnormal spermiogenesis ( J:342837 )

• abnormal residual cytoplasm is noted on the lumen side of elongated spermatids at stage VII

• cauda epididymal spermatozoa lack normal axoneme structures and exhibit a huge cytoplasm with abundant cytoplasmic contents including electron-dense material

• in step 6 spermatids, centrioles contact the cell surface, but not the nucleus; in step 8 spermatids, centrioles follow the sperm nucleus toward the cell surface at the opposite pole, as expected, but no cytoplasmic invagination around the axoneme is observed

• the flagellar compartment does not form properly and cytosol-exposed axonemal microtubules collapse during spermiogenesis

• ectopic localization of accessory structures, such as the fibrous sheath and outer dense fibers, is observed

abnormal spermiation ( J:342837 )

• sperm tails are hardly detectable in the lumen of seminiferous tubules

abnormal cauda epididymis morphology ( J:342837 )

• although some spermatozoa appear relatively normal, sloughed germ cells are detected within the epididymis

• a large number of frayed filaments -- identified as microtubules -- are noted in the cauda epididymis

male infertility ( J:342837 )

• no pups are born when male mice are naturally mated with wild-type females for 2 months

• however, vaginal plugs are observed