All Phenotypes Bpi<tm1.1Buls> MGI Mouse

immune system phenotype ( J:346703 )

N	• no difference in lymphocyte composition in spleens, peripheral lymph nodes and mesenteric lymph nodes in untreated mice

increased susceptibility to induced colitis ( J:346703 )

• at 7 and 12 days after dextran sodium sulfate (DSS) induced colitis disease scores are increased indicating more severe disease with more pronounced neutrophil influx

increased T cell number ( J:346703 )

• increase in CD3+CD4+CD44+ T cells in mesenteric lymph nodes of microbiota enriched mice compared to similarly enriched wild-type mice

decreased circulating interferon-gamma level ( J:346703 )

• on day 7 after DSS treatment, IFNG levels are significantly lower than in similarly treated controls

abnormal circulating interleukin level ( J:346703 )

• on day 7 after DSS treatment, IL22 levels are significantly lower than in similarly treated controls

enlarged mesenteric lymph nodes ( J:346703 )

• proximal mesenteric lymph nodes are enlarged with higher total cell numbers in microbiota enriched mice compared to similarly enriched wild-type mice

abnormal immune cell physiology ( J:346703 )

• polyclonal restimulation of mesenteric lymph node cells wiht alphaCD3 and alphaCD28 antibodies shows impaired cytokine responses for Ifng, IL22, and IL2

abnormal interleukin secretion ( J:346703 )

• decreased IL22 levels in CD3+CD4+ cells from mesenteric lymph nodes

• IL22 levels are also decreased in cells from peripheral lymph nodes but not in spleen cells

• ex vivo culturing of mesenteric lymph nodes cells fails to induce spontaneous IL22 secretion and secretion is reduced in the presence of activating antibodies