All Phenotypes Ighg2c<em1Wliu> MGI Mouse

decreased tumor growth/size ( J:322180 )

• mice show slower progression of inoculated MC38 colon adenocarcinoma tumor cells than wild-type controls

• tumor growth is inhibited during subsequent MC38-mOVA challenge

• mice transplanted with B16F10 melanoma tumor cells show decreased tumor progression

• adoptive transfer of MC38-primed B cells from mutant mice into B cell-deficient mice confers improved protection from tumor progression following MC38 tumor inoculation

• OVA-specific class-switched IgG2c+ B cells from mutant mice transferred into B cell-deficient mice exhibit stronger antitumor effects

decreased incidence of induced tumors ( J:322180 )

• tumor growth of mice inoculated with MC38 colon adenocarcinoma tumor cells or transplanted with B16F10 melanoma tumor cells is reduced

decreased incidence of tumors by chemical induction ( J:322180 )

• mice treated with azoxymethane/dextran sodium sulfate (AOM/DSS) to induce colitis-associated carcinoma, show reduced weight loss, decreased disease activity index, alleviated phenotypes of tumor-associated inflammation, reduced tumor burden, and ameliorated histopathological changes

• mice with AOM/DSS-induced colitis-associated carcinoma show larger tertiary lymphoid structure formation within colon tumors

• tumors from mice with AOM/DSS-induced CAC show increased transcriptional levels of chemokines CXCR5, CXCL12, CXCL13, CCL19, CCL21

• however, no significant difference is seen in the major histocompatibility complex (MHC) and costimulatory molecules CD86 on the cell surface of B cells in the tumor microenvironment, indicating no effect on priming function of B cells to activate T cells

decreased susceptibility to induced colitis ( J:322180 )

• mice treated with azoxymethane/dextran sodium sulfate (AOM/DSS) to induce colitis-associated carcinoma, show reduced weight loss and decreased disease activity index

abnormal plasma cell differentiation ( J:322180 )

• a higher percentage of class-switched IgG2c+ plasma cells are seen in the mesenteric lymph nodes, the colonic lamina propria of AOM/DSS-induced mice and the tumor-draining lymph nodes of MC38 tumor-bearing mice, indicating enhanced plasma cell differentiation in the tumor microenvironment

increased CD103-positive CD11b-low dendritic cell number ( J:322180 )

• dendritic cells (DCs,) especially CD103+ type I conventional DCs (key antigen-presenting cells that transport antigens to lymphoid structures and prime tumor-specific CD8+ T cells), are elevated in the TME of the MC38 tumor model mice

• however, no differences in tumor-infiltrating macrophages and NK cells are seen in the MC38 tumor model mice

increased germinal center B cell number ( J:322180 )

• in an ovalbumin (OVA) antigen-based immunization model, mice show a higher percentage of OVA-specific IgG2c+ germinal center B cells in the spleen in the tumor microenvironment

increased memory B cell number ( J:322180 )

• in an ovalbumin (OVA) antigen-based immunization model, mice show an increase in OVA-specific IgG2c+ memory B cells in the spleen and bone marrow, indicating promotion of class-switched memory B cells in the tumor microenvironment

increased CD8-positive, alpha-beta T cell number ( J:322180 )

• AOM/DSS-induced mice with colorectal cancer exhibit increased infiltration of CD8+ T cells in colon tumor specimens with no differences in CD4+ T cells, NK cells, and macrophages

• infiltration of CD8+ T Cells and IFN-gamma+ CD8+ T cells in the lamina propria and mesenteric lymph nodes is slightly increased in colon tumor specimens of mice with AOM/DSS-induced colorectal cancer

• MC38 tumor-bearing mice show increased amounts of CD8+ T cells within subcutaneous tumor tissues, and especially increased CD44^hiCD62L^loCD8+ effector T cells, with elevated levels of cytolytic IFN-gamma and granzyme B production, suggesting that tumor-infiltrating CD8+ T cells may help to limit tumor progression

increased effector memory CD8-positive, alpha-beta T cell number ( J:322180 )

• MC38 tumor-bearing mice show increased CD44^hiCD62L^loCD8+ effector T cells

increased IgG2c level ( J:322180 )

• in the MC38 transplantation model, mice show elevated amounts of MC38 cell-specific IgG2c antibodies compared to wild-type mice

• B cells from the tumor-draining lymph nodes secrete higher amounts of IgG2c upon restimulation with irradiated MC38 cells than B cells from wild-type mice

• upon AOM/DSS induction, levels of intestinal IgG2c antibodies are increased in colon explants, while levels of intestinal IgA, IgG1, IgG2b, and IgG3 antibodies are unaffected

• after immunization of OVA antigen combined with Th1-prone adjuvant, OVA-specific IgG2c antibody production is increased

abnormal macrophage physiology ( J:322180 )

• tumor-infiltrating macrophages from MC38-mOVA-inoculated mice exhibit increased in vivo tumor-phagocytosing activity

• IgG purified from OVA-immunized mice with MC38-mOVA induction show more potent bone marrow derived macrophage phagocytosis of antibody-targeted tumor cells

abnormal macrophage activation involved in immune response ( J:322180 )

• IgG purified from OVA-immunized mice with MC38-mOVA induction show more potent bone marrow derived macrophage activation and phagocytosis of antibody-targeted tumor cells

abnormal dendritic cell physiology ( J:322180 )

• purified IgG antibodies from OVA-immunized mice enhances the tumor antigen uptake activities of FLT3L-dendrtic cells