All Phenotypes 2310065F04Rik<tm1.1Boet> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet	involves: C57BL/6 * SJL	MGI:7645202
cn2	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet Ino80^tm1.1Jland/Ino80^tm1.1Jland Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCrl	MGI:7645256
cn3	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet Ino80^tm1.1Jland/Ino80^tm1.1Jland Pax7^tm1(cre)Mrc/Pax7⁺	involves: 129S1/Sv * 129X1/SvJ	MGI:7645255

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

increased body weight ( J:306156 )

• body weight is increased in adult mice but not in 8-week-old mice

• the increase in mass of skeletal muscle correlates with the increase in weight

muscle

abnormal skeletal muscle morphology ( J:306156 )

• skeletal muscles show an enlarged pool of adult muscle stem cells (satellite cells); muscle stem cells do not show a major change in the activation state under baseline conditions

• preparations of whole body skeletal muscle, tibialis anterior muscle, and soleus muscle show increased numbers of muscle stem cells

abnormal skeletal muscle fiber morphology ( J:306156 )

• soleus muscle shows increased numbers of myonuclei, but unchanged weight or cross-sectional area of myofibers

• 8-month-old mice show increased number of both myonuclei and increased myofiber cross-sectional area, indicating hypertrophy of the myofibers

increased skeletal muscle mass ( J:306156 )

increased skeletal muscle weight ( J:306156 )

• adults, but not 8-week-old mice, show an increase in skeletal muscle weight

skeletal muscle hypertrophy ( J:306156 )

• increase in myofiber cross-sectional area of skeletal muscle, indicating skeletal muscle hypertrophy

increased satellite cell number ( J:306156 )

• skeletal muscles show an enlarged pool of adult muscle stem cells (satellite cells)

• however, no reduction in the heterochromatin is seen, indicating that the increased number of muscle stem cells is not due to loss of quiescence of muscle stem cells

abnormal muscle physiology ( J:306156 )

• small, but significant, increase in number of Pax7+/MyoD+ nuclei in tibialis anterior (TA) muscle, indicating an increased number of spontaneously activated muscle stem cells

• mice show no differences from wild-type mice in muscle regeneration following TA muscle damage by cardiotoxin injection, however when regeneration is completed, the same increases in muscle stem cell numbers, EdU-incorporating muscle stem cells, and myofiber size is seen as before the damage in regenerated muscle, indicating stimulation of muscle stem cell proliferation and skeletal muscle growth during regeneration

increased skeletal muscle satellite cell proliferation ( J:306156 )

• mice show a great increase in EdU+/Pax7+ muscle stem cells, indicating enhanced proliferation of muscle stem cell-derived myoblasts which eventually increases the number of quiescent muscle stem cells

• however after proliferation, these muscle stem cells return to a quiescent state

cellular

increased skeletal muscle satellite cell proliferation ( J:306156 )

• however after proliferation, these muscle stem cells return to a quiescent state

Allelic Composition	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet Ino80^tm1.1Jland/Ino80^tm1.1Jland Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
2310065F04Rik^tm1.1Boet mutation (0 available); any 2310065F04Rik mutation (0 available)
Ino80^tm1.1Jland mutation (1 available); any Ino80 mutation (440 available)
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (39 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

muscle phenotype ( J:306156 )

N	• adult mice treated with tamoxifen show complete abrogation of the increased number of Pax7+ muscle stem cells (satellite cells) seen in homozygous 2310065F04Rik^tm1.1Boet mice

Allelic Composition	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet Ino80^tm1.1Jland/Ino80^tm1.1Jland Pax7^tm1(cre)Mrc/Pax7⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
2310065F04Rik^tm1.1Boet mutation (0 available); any 2310065F04Rik mutation (0 available)
Ino80^tm1.1Jland mutation (1 available); any Ino80 mutation (440 available)
Pax7^tm1(cre)Mrc mutation (3 available); any Pax7 mutation (39 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

growth/size/body region phenotype ( J:306156 )

N	• the increased body weight seen in homozygous 2310065F04Rik^tm1.1Boet mice is normalized

muscle

muscle phenotype ( J:306156 )

• mice show complete abrogation of the increased number of Pax7+ muscle stem cells (satellite cells) and of the increased EdU-labeled quiescent muscle stem cells, the increased tibialis anterior muscle weight, and the muscle hypertrophy and increased myonuclei numbers in fibers that are seen in homozygous 2310065F04Rik^tm1.1Boet mice

• mice do not show the general myofiber hypertrophy that is seen in homozygous 2310065F04Rik^tm1.1Boet mice 4 weeks after cardiotoxin-induced muscle damage

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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