Phenotypes associated with this allele

• Allele Symbol: Aspscr1^tm1.1Jsbo
• Allele Name: targeted mutation 1.1, Jonathan S Bogan
• Allele ID: MGI:7645890
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Aspscr1^tm1.1Jsbo/Aspscr1^tm1.1Jsbo Tg(Ckmm-cre)5Khn/0	B6.Cg-Aspscr1^tm1.1Jsbo Tg(Ckmm-cre)5Khn	MGI:7645894

Genotype
MGI:7645894

cn1

• Allelic Composition: Aspscr1^tm1.1Jsbo/Aspscr1^tm1.1Jsbo; Tg(Ckmm-cre)5Khn/0
• Genetic Background: B6.Cg-Aspscr1^tm1.1Jsbo Tg(Ckmm-cre)5Khn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased fatty acid oxidation ( J:308358 )

• ex vivo palmitate oxidation is reduced in soleus muscles indicating reduced fatty acid oxidation

decreased energy expenditure ( J:308358 )

• mice fed a high-fat diet have an overall 9% reduction in energy expenditure, which is more marked during light (resting) hours than during the dark hours

• when normalized to lean mass, energy expenditure is decreased by 6% in high-fat fed mice

• however, on a regular chow diet, energy expenditure is normal, even when light and dark hours are analyzed separately

increased susceptibility to diet-induced obesity ( J:308358 )

• mice fed a high-fat diet gain weight more rapidly than wild-type mice

• after 3 weeks on a high-fat diet, mice show increased body weight, fat mass, and lean mass, with increased percent fat mass and decreased percent lean mass

• however, body weight and composition and heart weight are unchanged in regular chow-fed mice

decreased carbon dioxide production ( J:308358 )

• mice fed a high-fat diet show reduced rates of carbon dioxide production

decreased oxygen consumption ( J:308358 )

• mice fed a high-fat diet show reduced rates of oxygen consumption

abnormal glucose homeostasis ( J:308358 )

• in fasting mice, whole-body glucose turnover is increased by 27%

decreased fasting circulating glucose level ( J:308358 )

increased fasting circulating glucose level ( J:308358 )

• mice fed a high-fat diet develop increased fasting plasma glucose levels

decreased circulating insulin level ( J:308358 )

• mice exhibit reduced fasting plasma insulin levels

increased circulating insulin level ( J:308358 )

• mice fed a high-fat diet develop increased fasting insulin levels

increased skeletal muscle glycogen level ( J:308358 )

• quadriceps glycogen content is increased 38% in mice fasted for 2 hours

increased insulin sensitivity ( J:308358 )

• homeostatic model assessment of insulin resistance (HOMA-IR), calculated from the product of fasting glucose and insulin concentrations, is reduced

increased skeletal muscle triglyceride level ( J:308358 )

• muscles from high-fat diet fed mice exhibit increased triglyceride content

growth/size/body

increased body fat mass ( J:308358 )

• in regular chow-fed mice housed under thermoneutral conditions

increased susceptibility to weight gain ( J:308358 )

• regular chow-fed mice housed under thermoneutral conditions (30 degrees C) show increased weight gain and gonadal white adipose tissue

increased susceptibility to diet-induced obesity ( J:308358 )

• mice fed a high-fat diet gain weight more rapidly than wild-type mice

• after 3 weeks on a high-fat diet, mice show increased body weight, fat mass, and lean mass, with increased percent fat mass and decreased percent lean mass

• however, body weight and composition and heart weight are unchanged in regular chow-fed mice

muscle

increased skeletal muscle cell glucose uptake ( J:308358 )

• muscle-specific fasting glucose uptake is increased 2-fold in gastrocnaemius and 1.8-fold in quadriceps

• however, heart-specific glucose uptake is unchanged

abnormal skeletal muscle morphology ( J:308358 )

• muscles from high-fat diet fed mice exhibit lipid droplets

abnormal skeletal muscle fiber mitochondrial morphology ( J:308358 )

• muscles from high-fat diet fed mice exhibit enlarged, swollen mitochondria, with disorganized cristae

increased skeletal muscle glycogen level ( J:308358 )

• quadriceps glycogen content is increased 38% in mice fasted for 2 hours

increased skeletal muscle triglyceride level ( J:308358 )

• muscles from high-fat diet fed mice exhibit increased triglyceride content

adipose tissue

increased white adipose tissue amount ( J:308358 )

• regular chow-fed mice housed under thermoneutral conditions show increased gonadal white adipose tissue

increased body fat mass ( J:308358 )

• in regular chow-fed mice housed under thermoneutral conditions

cellular

abnormal skeletal muscle fiber mitochondrial morphology ( J:308358 )

• muscles from high-fat diet fed mice exhibit enlarged, swollen mitochondria, with disorganized cristae

increased skeletal muscle cell glucose uptake ( J:308358 )

• muscle-specific fasting glucose uptake is increased 2-fold in gastrocnaemius and 1.8-fold in quadriceps

• however, heart-specific glucose uptake is unchanged

decreased fatty acid oxidation ( J:308358 )

• ex vivo palmitate oxidation is reduced in soleus muscles indicating reduced fatty acid oxidation

behavior/neurological

behavior/neurological phenotype ( J:308358 )

N • mice fed a high-fat diet show no difference in locomotor activity

increased fluid intake ( J:308358 )

• water intake is increased by 29%

increased food intake ( J:308358 )

• high-fat diet fed mice show a 22% increase in food intake

• however, no difference in food intake is seen in regular-chow fed mice