About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Dnmt3aem1Hsas
endonuclease-mediated mutation 1, Hiroyuki Sasaki
MGI:7646874
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Dnmt3aem1Hsas/Dnmt3aem1Hsas C57BL/6J-Dnmt3aem1Hsas MGI:7646977
ht2
 		Dnmt3aem1Hsas/Dnmt3a+ C57BL/6J-Dnmt3aem1Hsas MGI:7646978
cx3
 		Dnmt3aem1Hsas/Dnmt3aem1Hsas
Dnmt3lem1Hsas/Dnmt3lem1Hsas 		C57BL/6J-Dnmt3aem1Hsas Dnmt3lem1Hsas MGI:7647010


Genotype
MGI:7646977
hm1
Allelic
Composition		 Dnmt3aem1Hsas/Dnmt3aem1Hsas
Genetic
Background		 C57BL/6J-Dnmt3aem1Hsas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dnmt3aem1Hsas mutation (0 available); any Dnmt3a mutation (138 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal DNA methylation during gametogenesis ( J:347433 , J:349016 )
• global reduction of CG methylation to 17.6% in full-grown oocytes (FGOs) (J:347433)
• global reduction of non-CG methylation in full-grown oocytes (FGOs) (J:347433)
• global reduction of CG methylation to 66.3% in spermatozoa (J:347433)
• lack of processive methylation in growing and full-grown oocytes (J:349016)
abnormal imprinting ( J:347433 , J:349016 )
• reduction of maternal imprinting control region (ICR) methylation to 65-34% in full-grown oocytes (FGOs) (J:347433)
• stochastic loss of imprinting control region (ICR) methylation on maternal chromosomes in E10.5 embryos with crosses of homozygous mothers and wildtype fathers (J:349016)

mortality/aging
prenatal lethality, incomplete penetrance ( J:349016 )
• frequent embryo resorption at E14.5 and E18.5 with crosses of homozygous mothers and wildtype fathers

reproductive system
abnormal DNA methylation during gametogenesis ( J:347433 , J:349016 )
• global reduction of CG methylation to 17.6% in full-grown oocytes (FGOs) (J:347433)
• global reduction of non-CG methylation in full-grown oocytes (FGOs) (J:347433)
• global reduction of CG methylation to 66.3% in spermatozoa (J:347433)
• lack of processive methylation in growing and full-grown oocytes (J:349016)
abnormal pregnancy ( J:349016 )
• frequent embryo resorption at E14.5 and E18.5 with crosses of homozygous mothers and wildtype fathers
• small embryos at E10.5 with crosses of homozygous mothers and wildtype fathers
abnormal parturition ( J:349016 )
• low delivery rate (of stillborn pups) and extended pregnancy when mated with wildtype males




Genotype
MGI:7646978
ht2
Allelic
Composition		 Dnmt3aem1Hsas/Dnmt3a+
Genetic
Background		 C57BL/6J-Dnmt3aem1Hsas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dnmt3aem1Hsas mutation (0 available); any Dnmt3a mutation (138 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal imprinting ( J:349016 )
• stochastic loss of imprinting control region (ICR) methylation on maternal chromosomes in E10.5 embryos with crosses of homozygous mothers and wildtype fathers

mortality/aging
prenatal lethality, incomplete penetrance ( J:349016 )
• very few live pups born from foster mothers carrying zygotes from crosses of homozygous mothers and wildtype fathers
• frequent embryo resorption at E14.5 and E18.5 with crosses of homozygous mothers and wildtype fathers




Genotype
MGI:7647010
cx3
Allelic
Composition		 Dnmt3aem1Hsas/Dnmt3aem1Hsas
Dnmt3lem1Hsas/Dnmt3lem1Hsas
Genetic
Background		 C57BL/6J-Dnmt3aem1Hsas Dnmt3lem1Hsas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dnmt3aem1Hsas mutation (0 available); any Dnmt3a mutation (138 available)
Dnmt3lem1Hsas mutation (0 available); any Dnmt3l mutation (49 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
teratozoospermia ( J:347433 )
• severe impairment in motility and viability of spermatozoa obtained from epididymis
abnormal spermatid morphology ( J:347433 )
• reduced number of spermatids in seminiferous tubules
abnormal DNA methylation during gametogenesis ( J:347433 )
• global reduction of CG methylation to 6.2% in full-grown oocytes (FGOs)
• global reduction of CG methylation to 50.4% in spermatozoa
• global increase of CG methylation in spermatozoa and full-grown oocytes (FGOs)
abnormal imprinting ( J:347433 )
• reduction of maternal imprinting control region (ICR) methylation to 8-19% in full-grown oocytes (FGOs)

embryo
embryo phenotype ( J:347433 )
N
• viable
• born at approximately normal Mendelian ratios

endocrine/exocrine glands
abnormal seminiferous tubule morphology ( J:347433 )
• reduced number of spermatids

mortality/aging
mortality/aging ( J:347433 )
N
• viable

reproductive system
reproductive system phenotype ( J:347433 )
N
• normal overall ovary size and normal full-grown oocyte (FGO) morphology
teratozoospermia ( J:347433 )
• severe impairment in motility and viability of spermatozoa obtained from epididymis
abnormal spermatid morphology ( J:347433 )
• reduced number of spermatids in seminiferous tubules
abnormal DNA methylation during gametogenesis ( J:347433 )
• global reduction of CG methylation to 6.2% in full-grown oocytes (FGOs)
• global reduction of CG methylation to 50.4% in spermatozoa
• global increase of CG methylation in spermatozoa and full-grown oocytes (FGOs)
abnormal seminiferous tubule morphology ( J:347433 )
• reduced number of spermatids
abnormal fertility/fecundity ( J:347433 )
• no viable pups born when crossed with WT mice




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
07/05/2024
MGI 6.24 		The Jackson Laboratory