Phenotypes associated with this allele

• Allele Symbol: Eif4a1^tm1.1Bea
• Allele Name: targeted mutation 1.1, Douglas Strathdee
• Allele ID: MGI:7646942
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd79a^tm1(cre)Reth/Cd79a^+ Eif4a1^tm1.1Bea/Eif4a1^tm1.1Bea	involves: 129P2/OlaHsd * BALB/c * C57BL/6	MGI:7646987
cn2	Eif4a1^tm1.1Bea/Eif4a1^tm1.1Bea Tg(Fcer2a-cre)5Mbu/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:7646992

Genotype
MGI:7646987

cn1

• Allelic Composition: Cd79a^tm1(cre)Reth/Cd79a⁺; Eif4a1^tm1.1Bea/Eif4a1^tm1.1Bea
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased pro-B cell number ( J:344730 )

• greater than 20-fold reduction in pro B or Hardy Fraction B cells in the bone marrow

• remaining pro B cells have only a minor reduction in EIF4A1 and a 10-fold increase in EIF4A2

decreased B cell number ( J:344730 )

• almost complete loss of splenic B cells

immune system

decreased pro-B cell number ( J:344730 )

• greater than 20-fold reduction in pro B or Hardy Fraction B cells in the bone marrow

• remaining pro B cells have only a minor reduction in EIF4A1 and a 10-fold increase in EIF4A2

decreased B cell number ( J:344730 )

• almost complete loss of splenic B cells

Genotype
MGI:7646992

cn2

• Allelic Composition: Eif4a1^tm1.1Bea/Eif4a1^tm1.1Bea; Tg(Fcer2a-cre)5Mbu/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

hematopoietic system

hematopoietic system phenotype ( J:344730 )

N • normal numbers of splenic immature B cells

decreased B cell proliferation ( J:344730 )

• in a mixed bone marrow competitive chimera the number of Fo and MZ cells derived from null mice are reduced by 47% and 59%, respectively

• in culture the proliferation index is reduced to 1 compared to 3 in control cells

decreased germinal center B cell number ( J:344730 )

• 7 days after immunization with 4-hydroxy-3-nitrophenyl-acetyl conjugated to keyhole limpet hemocyanin germinal center B cells are almost completely absent with a 10-fold reduction compared to similarly treated control mice

• light zone and dark zone germinal center B cells are reduced by 9-fold and 15-fold, respectively

• reduction in B cells corresponds to a greater than 100-fold reduction in NP-specific IgG1 antibody secreting cells in the spleen

• remaining B cells have only a 40% reduction in EIF4A1

decreased IgG1 level ( J:344730 )

• decrease in both low- and high-affinity IgG1 antibodies following immunization

cellular

decreased B cell proliferation ( J:344730 )

• in a mixed bone marrow competitive chimera the number of Fo and MZ cells derived from null mice are reduced by 47% and 59%, respectively

• in culture the proliferation index is reduced to 1 compared to 3 in control cells

immune system

decreased B cell proliferation ( J:344730 )

• in a mixed bone marrow competitive chimera the number of Fo and MZ cells derived from null mice are reduced by 47% and 59%, respectively

• in culture the proliferation index is reduced to 1 compared to 3 in control cells

decreased germinal center B cell number ( J:344730 )

• 7 days after immunization with 4-hydroxy-3-nitrophenyl-acetyl conjugated to keyhole limpet hemocyanin germinal center B cells are almost completely absent with a 10-fold reduction compared to similarly treated control mice

• light zone and dark zone germinal center B cells are reduced by 9-fold and 15-fold, respectively

• reduction in B cells corresponds to a greater than 100-fold reduction in NP-specific IgG1 antibody secreting cells in the spleen

• remaining B cells have only a 40% reduction in EIF4A1

decreased IgG1 level ( J:344730 )

• decrease in both low- and high-affinity IgG1 antibodies following immunization