All Phenotypes Garin3<em1Osb> MGI Mouse

abnormal sperm head morphology ( J:350937 )

• 91.5% of sperm heads exhibit bluntness in the hook regions

abnormal acrosome morphology ( J:350937 )

• acrosome area of spermatozoa is larger

• however, no difference in acrosome reaction rates after incubation in capacitation medium is seen

asthenozoospermia ( J:350937 )

• straight line velocity, curvilinear velocity, and average path velocity of spermatozoa are reduced after 10 minutes of incubation in a capacitation medium and curvilinear velocity of spermatozoa is lower after 120 minutes of incubation

• however, the percentage of motile spermatozoa is comparable to wild-type

male infertility ( J:350937 )

• males completely fail to sire pups with wild-type females, and sperm cannot fertilize eggs in vivo

• in the presence of cumulus cells and intact zona pellucida, the in vitro fertilization rate of spermatozoa is decreased

• in the absence of cumulus cells, in vitro fertilization rate of spermatozoa is decreased

• however, the decreased fertilization rate of spermatozoa is rescued by removing both cumulus cells and zona pellucida

impaired sperm penetration of zona pellucida ( J:350937 )

• the decreased fertilization rate of spermatozoa is rescued by removing both cumulus cells and zona pellucida, indicating that zona pellucida penetration is impaired

impaired binding of sperm to zona pellucida ( J:350937 )

• the zona pellucida binding ability of spermatozoa is reduced, with about 31.3% of the binding ability compared to wild-type spermatozoa