Phenotypes associated with this allele

• Allele Symbol: Tmem268^em1Cheyy
• Allele Name: endonuclease-mediated mutation 1, Yingyu Chen
• Allele ID: MGI:7709327
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tmem268^em1Cheyy/Tmem268^em1Cheyy	involves: C57BL/6	MGI:7709328

Genotype
MGI:7709328

hm1

• Allelic Composition: Tmem268^em1Cheyy/Tmem268^em1Cheyy
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:350812 )

♂ • in a cecal ligation and puncture (CLP) model of sepsis mortality (43% compared to 0%) is significantly higher at 24h after CLP compared to similarly treated wild-type controls

♂ • at 231h after CLP only 14% of mice survive compared to 71% of wild-type controls

♂ • lethality following CLP is also increased when bone marrow from null mice is transplanted into lethally irradiated wild-type

immune system

immune system phenotype ( J:350812 )

N • proportion of B cells, T cells, monocytes, neutrophils, and macrophages in different tissues are comparable to controls

abnormal neutrophil cell number ( J:350812 )

♂ • decreased proportion of CD11b+Ly6G+ neutrophils in the peritoneal cavity following CLP; however, the proportion in the blood is increased

decreased macrophage cell number ( J:350812 )

♂ • decreased proportion of CD11b+F4/80+ macrophages in the peritoneal cavity following CLP

abnormal monocyte cell number ( J:350812 )

♂ • decreased proportion of CD11b+CD14+ monocytes in the peritoneal cavity following CLP; however, the proportion in the blood is increased

abnormal leukocyte physiology ( J:350812 )

abnormal leukocyte adhesion ( J:350812 )

• impaired adhesion of bone marrow derived macrophages to fibronectin and recombinant ICAM-1 following LPS stimulation

impaired leukocyte migration ( J:350812 )

• impaired adhesion and migration of bone marrow derived macrophages

impaired neutrophil phagocytosis ( J:350812 )

♂ • following LPS stimulation in the abdominal cavity

abnormal macrophage physiology ( J:350812 )

• enhanced degradation of CD11b via the endosomal-lysosomal pathway

impaired macrophage phagocytosis ( J:350812 )

♂ • following LPS stimulation in the abdominal cavity

increased circulating interferon-beta level ( J:350812 )

♂ • in males after CLP

increased circulating tumor necrosis factor level ( J:350812 )

♂ • in males after CLP

increased chemokine level ( J:350812 )

♂ • increased CCL2 serum levels after CLP

increased susceptibility to bacterial infection ( J:350812 )

♂ • at 24h after CLP bacterial burdens are higher in lung, liver, kidney, blood and peritoneal cavity

♂ • after CLP serum levels of proinflammatory cytokines are increased

♂ • severely damaged lung and kidney tissues and an increase in the number of apoptotic cells in the lungs after CLP

increased susceptibility to bacterial infection induced morbidity/mortality ( J:350812 )

♂ • in a cecal ligation and puncture (CLP) model of sepsis mortality (43% compared to 0%) is significantly higher at 24h after CLP compared to similarly treated wild-type controls

♂ • at 231h after CLP only 14% of mice survive compared to 71% of wild-type controls

♂ • lethality following CLP is also increased when bone marrow from null mice is transplanted into lethally irradiated wild-type

homeostasis/metabolism

increased circulating interferon-beta level ( J:350812 )

♂ • in males after CLP

increased circulating tumor necrosis factor level ( J:350812 )

♂ • in males after CLP

increased chemokine level ( J:350812 )

♂ • increased CCL2 serum levels after CLP

cellular

abnormal leukocyte adhesion ( J:350812 )

• impaired adhesion of bone marrow derived macrophages to fibronectin and recombinant ICAM-1 following LPS stimulation

impaired leukocyte migration ( J:350812 )

• impaired adhesion and migration of bone marrow derived macrophages

abnormal vesicle-mediated transport ( J:350812 )

♂ • following LPS stimulation, phagocytosis by macrophages, monocytes, and neutrophils in the abdominal cavity is reduced

impaired macrophage phagocytosis ( J:350812 )

♂ • following LPS stimulation in the abdominal cavity

impaired neutrophil phagocytosis ( J:350812 )

♂ • following LPS stimulation in the abdominal cavity

hematopoietic system

abnormal neutrophil cell number ( J:350812 )

♂ • decreased proportion of CD11b+Ly6G+ neutrophils in the peritoneal cavity following CLP; however, the proportion in the blood is increased

decreased macrophage cell number ( J:350812 )

♂ • decreased proportion of CD11b+F4/80+ macrophages in the peritoneal cavity following CLP

abnormal monocyte cell number ( J:350812 )

♂ • decreased proportion of CD11b+CD14+ monocytes in the peritoneal cavity following CLP; however, the proportion in the blood is increased

abnormal leukocyte physiology ( J:350812 )

abnormal leukocyte adhesion ( J:350812 )

• impaired adhesion of bone marrow derived macrophages to fibronectin and recombinant ICAM-1 following LPS stimulation

impaired leukocyte migration ( J:350812 )

• impaired adhesion and migration of bone marrow derived macrophages

impaired neutrophil phagocytosis ( J:350812 )

♂ • following LPS stimulation in the abdominal cavity

abnormal macrophage physiology ( J:350812 )

• enhanced degradation of CD11b via the endosomal-lysosomal pathway

impaired macrophage phagocytosis ( J:350812 )

♂ • following LPS stimulation in the abdominal cavity