Analysis Tools
immune system
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• neonatal and adult mice administered with a minimal stimulatory ssDNA mimic (CGT ODN) intracellularly via lipid nanoparticles to the liver show almost completely abolished all CGT ODN-induced responses, with almost no activation of cytokine expression, no hepatocyte necrosis or lobular architecture disruption in the liver, no elevation of serum alanine aminotransferase and aspartate aminotransferase, and no septic shock at higher concentrations as seen in treated wild-type mice
• injection of juvenile mice with adeno-associated virus type 2 (AAV2) gDNA encompassed by lipid nanoparticles does not induce severe immune responses that are seen in wild-type controls, with only a slight reduction in survival compared to 0% survival after 1.5 days of treatment in controls and reduced expression of Ifnb1, Tnf, and Cxcl2
• however, mice are healthy and fertile under specific pathogen-free conditions
• mouse adult fibroblasts and lung fibroblasts show inhibition of HIV6441-induced Ifnb1 expression
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• primary bone marrow-derived macrophages (BMDMs) show inhibition of Ifnb1, Il6, and Cxcl2 activation by stimulatory CGT-motif single-stranded oligodeoxynucleotides (CGT-ODNs), ODN-H and HIV6441, but not by poly(dA;dT), poly(I:C) or lipopolysaccharide
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hematopoietic system
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• primary bone marrow-derived macrophages (BMDMs) show inhibition of Ifnb1, Il6, and Cxcl2 activation by stimulatory CGT-motif single-stranded oligodeoxynucleotides (CGT-ODNs), ODN-H and HIV6441, but not by poly(dA;dT), poly(I:C) or lipopolysaccharide
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