Phenotypes associated with this allele

• Allele Symbol: Olah^em1Kedk
• Allele Name: endonuclease-mediated mutation 1, Katherine Kedzierska
• Allele ID: MGI:7720263
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Olah^em1Kedk/Olah^em1Kedk	involves: C57BL/6	MGI:7720837

Genotype
MGI:7720837

hm1

• Allelic Composition: Olah^em1Kedk/Olah^em1Kedk
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to Orthomyxoviridae infection induced morbidity/mortality ( J:353571 )

• following infection with 10^5 PFU H3N2; X31 influenza strain all mice survive, while about half of similarly infected wild-type mice die

• transfer of wild-type bone marrow cells into homozygous mice negates the protection from death following infection

renal/urinary system

abnormal urethra morphology ( J:353571 )

• prominent dilation and epithelial hyperplasia

abnormal urethra urothelium morphology ( J:353571 )

• epithelial hyperplasia

immune system

spleen hyperplasia ( J:353571 )

• mild follicular hyperplasia with germinal center in the white pulp

abnormal macrophage morphology ( J:353571 )

• increased number of lipid droplets in uninfected macrophages

abnormal macrophage physiology ( J:353571 )

• following infection fewer macrophages express viral proteins and differences in antigen expression are seen from 4h post infection

• viral titers of West Nile virus and Kunjin virus are significantly reduced

• following infection increase in lipid droplets is reduced compared to wild-type mice

decreased susceptibility to Orthomyxoviridae infection ( J:353571 )

• following infection with 10^4 PFU H3N2; X31 influenza strain, mice lose less weight over 10 days compared to similarly infected wild-type mice

• at 1 day post infection pro-inflammatory cytokines are reduced in lungs and bronchoalveolar lavage with a commensurate decrease in cell numbers at the infection site

• decreased numbers of alveolar and interstitial macrophages, neutrophils, gamma delta T cells, natural killer and natural killer T cells in the lungs at 1 day but not 2 or 3 days post infection

• lipid drop accumulation in the lungs after infection decreases in homozygous mice in contrast to wild-type mice where lipid droplets increase post infection

• equivalent numbers of CD8+ and CD4+ T cells are detected in the lungs post infection, with most CD8+ T cells showing effector phenotypes

decreased susceptibility to Orthomyxoviridae infection induced morbidity/mortality ( J:353571 )

• following infection with 10^5 PFU H3N2; X31 influenza strain all mice survive, while about half of similarly infected wild-type mice die

• transfer of wild-type bone marrow cells into homozygous mice negates the protection from death following infection

homeostasis/metabolism

abnormal lipid level ( J:353571 )

• decrease in 69 distinct lipids and increase in 13 others

• downregulated lipids are ~25% cardiolipins and their derivatives and 37% lysophosphatidylethanolamines

decreased fatty acids level ( J:353571 )

• decreased levels of oleic, palmitoleic palmitic, and myristic acid

hematopoietic system

spleen hyperplasia ( J:353571 )

• mild follicular hyperplasia with germinal center in the white pulp

extramedullary hematopoiesis ( J:353571 )

• mild extramedullary hematopoiesis and hemosiderin laden macrophages identified in the red pulp

abnormal macrophage morphology ( J:353571 )

• increased number of lipid droplets in uninfected macrophages

abnormal macrophage physiology ( J:353571 )

• following infection fewer macrophages express viral proteins and differences in antigen expression are seen from 4h post infection

• viral titers of West Nile virus and Kunjin virus are significantly reduced

• following infection increase in lipid droplets is reduced compared to wild-type mice

growth/size/body

spleen hyperplasia ( J:353571 )

• mild follicular hyperplasia with germinal center in the white pulp