All Phenotypes Cox6b2<em1Osb> MGI Mouse

reproductive system phenotype ( J:345850 )

N	• female homozygotes are fertile • although subfertile, male homozygotes show no significant differences in gross testis morphology, average testis weight, testis histology, or sperm mitochondrial function relative to wild-type controls

decreased sperm progressive motility ( J:345850 )

• cauda epididymal spermatozoa show a significantly lower progressive sperm rate (%) at 120 min, but not at 10 min, after incubation in TYH capacitation medium

asthenozoospermia ( J:345850 )

• cauda epididymal spermatozoa show a significantly lower motility (%) at 120 min, but not at 10 min, after incubation in TYH capacitation medium

• in addition, spermatozoa exhibit reduced VAP (average-path velocity) at 120-min, VCL (curvilinear velocity) at 10-min, and VSL (straight-line velocity) at 120-min incubation

• however, flagellar waveforms are normal at 10 min after incubation

decreased litter size ( J:345850 )

• when individual male homozygotes are housed with wild-type females for 2 months, the average litter size is significantly lower than that for wild-type males

reduced male fertility ( J:345850 )

• male homozygous are subfertile; they sometimes fail to produce pups or produce only a few pups after mating

cellular phenotype ( J:345850 )

N	• under non-capacitation conditions, cauda epididymal sperm show a normal oxygen consumption rate (OCR) with no differences in basal respiration, ATP production, proton leak, maximal respiration or spare capacity relative to wild-type sperm, indicating normal sperm mitochondrial function