All Phenotypes Hnrnph1<tm1.1Nju> MGI Mouse

oligozoospermia ( J:333262 )

• adults show reduced numbers of spermatocytes and spermatids and no detectable mature spermatozoa in seminiferous tubules and cauda epididymis

abnormal spermatid morphology ( J:333262 )

• decrease in spermatids in adults

abnormal spermatocyte morphology ( J:333262 )

• decrease in spermatocytes in adults

abnormal male meiosis ( J:333262 )

• a higher proportion of spermatocytes show an abnormal gamma-H2A.X distribution, indicating the DNA damage response remains active in synapsed homologs of pachytene spermatocytes

• pachytene spermatocytes show abnormal double-strand DNA break (DSB) repair

• pachytene and diplotene spermatocytes are reduced indicating developmental retardation of pachytene spermatocytes

increased male germ cell apoptosis ( J:333262 )

• the number of apoptotic cells in testis is increased even at the early P14 stage, suggesting dystrophy of the developing spermatocytes during the first wave of spermatogenesis

abnormal seminiferous tubule morphology ( J:333262 )

• F-actin is disorganized in seminiferous tubules

abnormal Sertoli cell barrier morphology ( J:333262 )

• abnormal Sertoli cell morphology and blood-testis barrier structure with large irregular cavities is seen in the seminiferous epithelium

• basal tight junction proteins (N-cadherin and beta-catenin) and ectoplasmic specialization protein (ZO-1) are found beyond the basement membrane of seminiferous tubules and diffusely present at the blood-testis barrier, extending towards the lumen

abnormal Sertoli cell morphology ( J:333262 )

• cytoskeleton of Sertoli cells shows disruption of microtubular arrangement and actin organization

• length of vimentin filaments in Sertoli cells in seminiferous tubules is shorter

abnormal ectoplasmic specialization morphology ( J:333262 )

• the structure of apical ectoplasmic specialization is disrupted

small seminiferous tubules ( J:333262 )

• developing testes show decreased diameter of seminiferous tubules from P21 onwards

small testis ( J:333262 )

decreased testis weight ( J:333262 )

• ratio of testis weight to body weight is reduced from P21 onwards

abnormal Sertoli cell barrier function ( J:333262 )

• biotin tracer permeates into seminiferous tubules indicating disrupted blood-testis barrier

arrest of spermatogenesis ( J:333262 )

• spermatogenesis is arrested in step 15 spermatids

• however, development and differentiation of spermatogonia both in juvenile and adult mice are not affected

abnormal epididymis morphology ( J:333262 )

• a large number of round spermatids and a few spermatocytes are prematurely sloughed into cauda epididymis at P28 and P60

male infertility ( J:333262 )

• males never produce any pups