Analysis Tools
reproductive system
azoospermia
(
J:308739
)
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• no sperm are detected in the caudal epididymis
• epididymis shows only a few round-shaped cells with large nuclei that are likely immature spermatids and no mature spermatozoa
• seminiferous tubules exhibit non-obstructive azoospermia characterized by primary spermatocytes and few abnormal haploid cells
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• spermatocytes from metaphase spreads frequently show univalents at metaphase I, indicating compromised crossover formation in spermatocytes
• marker analysis indicates impaired meiotic recombination
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• testes predominantly contains diploid and tetraploid cells, with a few haploid cells compared to predominantly haploid cells in wild-type testes, indicating meiotic arrest
• tubules show an accumulation of primary spermatocytes and contain no round spermatids at 20 postnatal days and no elongating spermatids are seen in testes at 30 postnatal days and instead an accumulation of primary spermatocytes, in particular those in pachynema, are seen
• the diplotene and diakinesis stages of prophase I are almost lost, indicating meiotic arrest at the pachytene stage
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• an average of 30% of spermatocytes are apoptotic in testes compared to 6% in wild-type and an average of 9.3 apoptotic cells per tubule occurs in testes compared to approximately 1.5 for wild-type
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small testis
(
J:308739
)
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• males mated with wild-type females produce no litters
• however, no histologic differences in spermatogonia, Sertoli cells or interstitial tissues of testis
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endocrine/exocrine glands
small testis
(
J:308739
)
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cellular
azoospermia
(
J:308739
)
|
|
• no sperm are detected in the caudal epididymis
• epididymis shows only a few round-shaped cells with large nuclei that are likely immature spermatids and no mature spermatozoa
• seminiferous tubules exhibit non-obstructive azoospermia characterized by primary spermatocytes and few abnormal haploid cells
|
|
|
• spermatocytes from metaphase spreads frequently show univalents at metaphase I, indicating compromised crossover formation in spermatocytes
• marker analysis indicates impaired meiotic recombination
|
|
|
• testes predominantly contains diploid and tetraploid cells, with a few haploid cells compared to predominantly haploid cells in wild-type testes, indicating meiotic arrest
• tubules show an accumulation of primary spermatocytes and contain no round spermatids at 20 postnatal days and no elongating spermatids are seen in testes at 30 postnatal days and instead an accumulation of primary spermatocytes, in particular those in pachynema, are seen
• the diplotene and diakinesis stages of prophase I are almost lost, indicating meiotic arrest at the pachytene stage
|
|
|
• an average of 30% of spermatocytes are apoptotic in testes compared to 6% in wild-type and an average of 9.3 apoptotic cells per tubule occurs in testes compared to approximately 1.5 for wild-type
|
