Analysis Tools
immune system
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• most cells cells contain large granules with extensive electron lucent periphery and internal material
• lucent granules only rarely contain the classical bar-like crystalloid core
• numbers of granules with normal morphology are 4-fold lower compared to wild-type cells
• granularity of newly generated eosinophils is higher than in older null cells
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• flow cytometry side-scatter for Siglec-F+ eosinophils is very low
• under allergic conditions blood eosinophil numbers increases less than in similarly treated controls
• in lethally irradiated mice reconstituted with wild-type and null cells, wild-type eosinophils outnumber null eosinophils
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• BrdU incorporation indicates that turnover of eosinophils is increased
• in response to ovalbumin-specific lung inflammation few eosinophils with high side-scatter are recruited and instead Siglec F+ cells with reduced granularity are present
• increase in the incidence of early apoptotic eosinophils in the lungs
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• during the acute phase of allergen-induced airway infiltration peribronchiolar infiltration of eosinophils ans mucus production by hyperplastic goblet cells are significantly reduced
• during the chronic phase sub-epithelial matrix deposition is significantly attenuated
• acutely allergen exposed mice have lower levels of TNF-alpha, IFNG, and IL-13 compared to similarly treated controls
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• fail to clear infection with Brugia malayi at 28 days post infection unlike wild-type controls
• despite persistence of the infection the total numbers of leukocytes, eosinophils, and Epx activity are reduced compared to controls; however, levels of IFNG and microfilariae-specific IgE are increased in sera and BALF at 28 days post infection
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cellular
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• increase in the incidence of early apoptotic eosinophils in the lungs
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homeostasis/metabolism
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• in response to ovalbumin-specific lung inflammation total inflammatory cell infiltrate in the airways and accumulation of eosinophils is about 70% lower compared to similarly treated wild-type controls
• however, neutrophil, dendritic cell, and other leukocyte numbers are similar to controls
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hematopoietic system
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• most cells cells contain large granules with extensive electron lucent periphery and internal material
• lucent granules only rarely contain the classical bar-like crystalloid core
• numbers of granules with normal morphology are 4-fold lower compared to wild-type cells
• granularity of newly generated eosinophils is higher than in older null cells
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• flow cytometry side-scatter for Siglec-F+ eosinophils is very low
• under allergic conditions blood eosinophil numbers increases less than in similarly treated controls
• in lethally irradiated mice reconstituted with wild-type and null cells, wild-type eosinophils outnumber null eosinophils
|
|
• BrdU incorporation indicates that turnover of eosinophils is increased
• in response to ovalbumin-specific lung inflammation few eosinophils with high side-scatter are recruited and instead Siglec F+ cells with reduced granularity are present
• increase in the incidence of early apoptotic eosinophils in the lungs
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