Phenotypes associated with this allele

• Allele Symbol: Litchi^em1Jnch
• Allele Name: endonuclease-mediated mutation 1, Jun-An Chen
• Allele ID: MGI:7868147
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Litchi^em1Jnch/Litchi^em1Jnch	involves: C57BL/6J	MGI:8161517

Genotype
MGI:8161517

hm1

• Allelic Composition: Litchi^em1Jnch/Litchi^em1Jnch
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:345795 )

N • at E12.5, embryos show normal numbers of motor neuron (MN) progenitors (Olig2+), generic MNs (Isl1/2+), and MN subtypes (Lhx3+ and Foxp1+) in the spinal cord relative to wild-type controls

delayed axon extension ( J:345795 )

• Sholl analysis of primary motor neurons (MNs) shows a delay in the timing of neurite growth relative to wild-type MNs

abnormal dendrite arborization pattern ( J:345795 )

• on day 3 of in vitro culture (DIV-3), primary motor neurons (MNs) harvested from the spinal cord show significantly fewer intersections at ~20-120 um from the cell body, indicating reduced dendritic complexity

abnormal motor neuron dendrite morphology ( J:345795 )

• on day 3 of in vitro culture (DIV-3), primary motor neurons (MNs) harvested from the spinal cord show reduced dendritic complexity relative to wild-type MNs

behavior/neurological

behavior/neurological phenotype ( J:345795 )

N • at 1.5 months of age, mice exhibit normal exploratory behavior (total distance traveled over 60 min) in the open field test

impaired coordination ( J:345795 )

• at 1.5 months of age, mice exhibit a significant decrease in the latency-to-fall (sec) in a rotarod test

decreased grip strength ( J:345795 )

• at 1.5 months of age, mice exhibit significantly reduced forelimb grip strength relative to wild-type controls

muscle

muscle weakness ( J:345795 )

• adult mice exhibit muscle weakness

cellular

delayed axon extension ( J:345795 )

• Sholl analysis of primary motor neurons (MNs) shows a delay in the timing of neurite growth relative to wild-type MNs

mortality/aging

mortality/aging ( J:345795 )

N • mice are born in expected Mendelian ratios, are viable after birth, and exhibit a normal lifespan relative to wild-type controls

growth/size/body

growth/size/body region phenotype ( J:345795 )

N • at 1.5 months of age, mice appear grossly normal by X-ray micro-CT scanning and show normal body weight relative to wild-type controls