Analysis Tools
mortality/aging
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• mutant embryos exhibit early post-implantation lethality, with rapid resorption occurring during the gastrulation period
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cellular
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• at E6.75 and E7.5, cells with pycnotic nuclei and condensed chromatin are detected throughout the mutant embryos
• TUNEL-positive apoptotic cells are most abundant in the extra-embryonic region of E6.75 and E7.5 mutant embryos
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embryo
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• at E6.75 and E7.5, cells with pycnotic nuclei and condensed chromatin are detected throughout the mutant embryos
• TUNEL-positive apoptotic cells are most abundant in the extra-embryonic region of E6.75 and E7.5 mutant embryos
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• mutant embryos exhibit a developmental arrest at E4.5, at the egg-cylinder stage
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• at E7.5, mutant embryos are smaller and grossly abnormal relative to wild-type
• at E8.5 and E9.5, mutant embryos undergo resorption and are reduced to small masses of haemorrhagic tissue
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• at E6.75, many cells display nuclear and cytoplasmic features of necrosis
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• during resorption, the extraembryonic region of the conceptus is severely reduced and lacks distinct exocoelomic and chorionic cavities
• in contrast, the ectodermal, mesodermal and endodermal cell layers remain relatively unaffected
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growth/size/body
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• at E7.5, mutant embryos are smaller and grossly abnormal relative to wild-type
• at E8.5 and E9.5, mutant embryos undergo resorption and are reduced to small masses of haemorrhagic tissue
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homeostasis/metabolism
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• Perls staining indicates absence of iron-specific staining in mutant E6.5-E8.5 embryos
• also, no iron deposits are detected in resorbed E6.75 embryos, either in mitochondria or in the cytosol, suggesting that cell death is not due to abnormal iron accumulation
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