Phenotypes Associated with This Genotype

Genotype
MGI:3038845

hm1

• Allelic Composition: Adcy10^tm1Lex/Adcy10^tm1Lex
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

teratozoospermia ( J:100583 )

♂

hairpin sperm flagellum ( J:100583 )

♂ • over time, even cAMP analog-rescued mutant sperm fold back 180 degrees until the head and midpiece form a hairpin configuration with the rest of the sperm flagellum

kinked sperm flagellum ( J:100583 )

♂ • even after motility is restored with a cAMP analogue, ~100% of mutant sperm show flagellar angularity

♂ • the flagellum begins to bend at the annulus (the midpiece-principal piece junction) within 10 min of isolation

abnormal sperm motility ( J:100583 )

♂ • sperm fail to become hyperactive even after motility is restored with cAMP

asthenozoospermia ( J:88649 , J:100583 )

♂ • mutant sperm appear able to achieve no forward movement and produce only a trembling movement of the midpiece (J:88649)

♂ • motility could be restored in vitro by loading sperm with a cAMP analogue (J:88649)

♂ (J:100583)

impaired sperm capacitation ( J:100583 )

♂ • even cAMP analog-rescued mutant sperm are unable to fertilize eggs in vitro

male infertility ( J:88649 , J:100583 )

♂ (J:88649)

♂ (J:100583)

abnormal fertilization ( J:88649 , J:100583 )

• in an in vitro fertilization assay mutant sperm can induce cumulus release but do not produce any two-cell stage embryos (J:88649)

• restoration of sperm motility has little effect on fertility (J:100583)

cellular

teratozoospermia ( J:100583 )

♂

hairpin sperm flagellum ( J:100583 )

♂ • over time, even cAMP analog-rescued mutant sperm fold back 180 degrees until the head and midpiece form a hairpin configuration with the rest of the sperm flagellum

kinked sperm flagellum ( J:100583 )

♂ • even after motility is restored with a cAMP analogue, ~100% of mutant sperm show flagellar angularity

♂ • the flagellum begins to bend at the annulus (the midpiece-principal piece junction) within 10 min of isolation

abnormal sperm motility ( J:100583 )

♂ • sperm fail to become hyperactive even after motility is restored with cAMP

asthenozoospermia ( J:88649 , J:100583 )

♂ • mutant sperm appear able to achieve no forward movement and produce only a trembling movement of the midpiece (J:88649)

♂ • motility could be restored in vitro by loading sperm with a cAMP analogue (J:88649)

♂ (J:100583)