Phenotypes Associated with This Genotype

Genotype
MGI:3528062

cx5

• Allelic Composition: Gzma^tm1Simn/Gzma^tm1Simn; Gzmb^tm1Ley/Gzmb^tm1Ley
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:44204 )

N • in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice induce lysis in various target cells, at levels and with kinetics similar to those of single mutant or C57BL/6 mice

abnormal leukocyte physiology ( J:44204 )

• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice fail to induce apoptotic nuclear damage in target cells

• in double mutants, Tc/NK-mediated target cell DNA fragmentation is undetectable, even after extended incubation periods (10 h)

• in contrast, Tc/NK-mediated target cell DNA fragmentation is normal in Gzma^tm1Simn and only impaired in Gzmb^tm1Ley mice in short-term (2-4 h), but not long-term (4-10 h), nucleolytic assays

hematopoietic system

abnormal leukocyte physiology ( J:44204 )

• in vitro- and ex vivo-derived cytotoxic T cells and NK cells from double mutant mice fail to induce apoptotic nuclear damage in target cells

• in double mutants, Tc/NK-mediated target cell DNA fragmentation is undetectable, even after extended incubation periods (10 h)

• in contrast, Tc/NK-mediated target cell DNA fragmentation is normal in Gzma^tm1Simn and only impaired in Gzmb^tm1Ley mice in short-term (2-4 h), but not long-term (4-10 h), nucleolytic assays