Phenotypes Associated with This Genotype

Genotype
MGI:3618699

• Allelic Composition: Tg(TcraAI4)1Dvs/0; Tg(TcrbAI4)1Dvs/0
• Genetic Background: NOD-Tg(TcraAI4)1Dvs Tg(TcrbAI4)1Dvs

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal CD4-positive, alpha beta T cell morphology ( J:93553 )

• T cells expressing the transgenes comprise ~35% of the total peripheral blood lymphocyte population, a significant fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage

abnormal CD8-positive, alpha beta T cell morphology ( J:94192 )

• only transgene expressing CD8+ T cells from primary splenocytes expand when cultured in presence of islets from NOD-scid donors

abnormal cytotoxic T cell physiology ( J:94192 )

• transgenic CTLs generated in culture on islets exhibit cytotoxicity against a NOD-derived beta cell adenoma cell line and this is augmented when adenoma cells are pretreated with IFNG

increased susceptibility to autoimmune diabetes ( J:94192 )

• female NOD mice carrying both transgenes develop IDDM at a greatly increased rate compared to nontransgenic controls with disease appearing as early as 3 weeks of age

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:93553 )

• T cells expressing the transgenes comprise ~35% of the total peripheral blood lymphocyte population, a significant fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage

abnormal CD8-positive, alpha beta T cell morphology ( J:94192 )

• only transgene expressing CD8+ T cells from primary splenocytes expand when cultured in presence of islets from NOD-scid donors

abnormal cytotoxic T cell physiology ( J:94192 )

• transgenic CTLs generated in culture on islets exhibit cytotoxicity against a NOD-derived beta cell adenoma cell line and this is augmented when adenoma cells are pretreated with IFNG

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:93553