Phenotypes Associated with This Genotype

Genotype
MGI:3622794

• Allelic Composition: Adora2a^tm1Jfc/Adora2a^tm1Jfc
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired behavioral response to addictive substance ( J:99612 , J:107988 )

• no significant increase in wakefulness is seen in homozygotes after injection of 15 mg/kg, 10 mg/kg, or 5 mg/kg caffeine, unlike wild-type mice (J:99612)

• however, baseline circadian sleep-wake profiles are identical to wild-type (J:99612)

• single exposure amphetamine or cocaine stimulated increases in locomotor activity are reduced compared to wild-type mice (J:107988)

• however, amphetamine stimulated increases in fine movement are not significantly different from wild-type (J:107988)

decreased locomotor activity ( J:73594 , J:107988 )

• reduced spontaneous activity most prominently in the dark cycle; however normal locomotor circadian rhythms are seen (J:107988)

homeostasis/metabolism

decreased susceptibility to ischemic brain injury ( J:73594 )

• 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice

decreased cerebral infarct size ( J:73594 )

• after middle cerebral arterial occlusion total infarct volume is reduced by 26% using hematoxylin and eosin staining or by 77% using TTC staining (marker of intact cellular metabolism) compared to wild-type mice

nervous system

nervous system phenotype ( J:73594 , J:107988 )

N • morphology of cortex and striatum appears normal (J:73594)

N • dopaminergic innervation is normal (J:107988)

decreased susceptibility to ischemic brain injury ( J:73594 )

• 24 hours after middle cerebral arterial occlusion neurological deficit scores for homozygotes are reduced by 50-60% compared to wild-type mice

decreased cerebral infarct size ( J:73594 )

• after middle cerebral arterial occlusion total infarct volume is reduced by 26% using hematoxylin and eosin staining or by 77% using TTC staining (marker of intact cellular metabolism) compared to wild-type mice

cardiovascular system

cardiovascular system phenotype ( J:73594 )

N • basal mean arterial blood pressure, absolute blood flow, and cortical cerebral blood flow before, during. and after middle cerebral arterial occlusion is similar to wild-type mice

vision/eye

vision/eye phenotype ( J:164077 )

N • normal growth in the anterior segment and normal pupil size

abnormal sclera morphology ( J:164077 )

• sclera contains denser collagen fibrils with reduced diameter

myopia ( J:164077 )

• mice develop greater myopia than wild-type mice, that is associated with increases in vitreous chamber depth and axial length from P28 to P56

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
myopia		DOID:11830	OMIM:160700 OMIM:255500 OMIM:300613 OMIM:310460 OMIM:603221 OMIM:608367 OMIM:608474 OMIM:608908 OMIM:609256 OMIM:609257 OMIM:609258 OMIM:609259 OMIM:609994 OMIM:609995 OMIM:610320 OMIM:612554 OMIM:612717 OMIM:613959 OMIM:613969 OMIM:614166 OMIM:614167 OMIM:615420 OMIM:615431 OMIM:615946	J:164077