Phenotypes Associated with This Genotype

Genotype
MGI:3687163

• Allelic Composition: Rag1^tm1Mom/Rag1^tm1Mom; Tg(TcraBDC12-4.1)10Jos/0; Tg(TcrbBDC12-4.1)82Gse/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * FVB * NOD

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating glucose level ( J:111874 )

• mice are diabetic; diabetes assessed by blood glucose measures >250 mg/dl on two consectutive measurements

endocrine/exocrine glands

decreased pancreatic beta cell number ( J:111874 )

• diabetic mice have nearly complete destruction of insulin producing cells

decreased thymus weight ( J:111874 )

• thymus weight is variable, with some being normal and others having very low weight

• transgene expression in Rag1-deficient mice increases thymus cellularity and thymus weight almost to levels seen in NOD controls

immune system

decreased thymus weight ( J:111874 )

• thymus weight is variable, with some being normal and others having very low weight

• transgene expression in Rag1-deficient mice increases thymus cellularity and thymus weight almost to levels seen in NOD controls

increased double-positive T cell number ( J:111874 )

• mice have greater percentage of double-positive T cells (93%) in the thymus compared to other genotypes

decreased lymphocyte cell number ( J:111874 )

• mice are lymphopenic compared to Rag1-sufficient transgenic mice and control NOD animals; number of lymphocytes in peripheral blood is lower than in transgenic Rag1-sufficient mice both in percentage of total white blood cell count and absolute lymphocyte count

decreased CD4-positive, alpha-beta T cell number ( J:111874 )

• mice have lower percentage of single positive CD4+ T cells (3%) in the thymus compared to other genotypes

increased susceptibility to autoimmune diabetes ( J:111874 )

• mice develop spontaneous diabetes aged 4-32 weeks; diabetes develops only in mice with at least 1 copy of H-2^g7 (incidence is 50% with homozygosity, 15% in heterozygotes); both sexes of transgenic mice are equally affected

hematopoietic system

decreased thymus weight ( J:111874 )

• thymus weight is variable, with some being normal and others having very low weight

• transgene expression in Rag1-deficient mice increases thymus cellularity and thymus weight almost to levels seen in NOD controls

increased double-positive T cell number ( J:111874 )

• mice have greater percentage of double-positive T cells (93%) in the thymus compared to other genotypes

decreased lymphocyte cell number ( J:111874 )

• mice are lymphopenic compared to Rag1-sufficient transgenic mice and control NOD animals; number of lymphocytes in peripheral blood is lower than in transgenic Rag1-sufficient mice both in percentage of total white blood cell count and absolute lymphocyte count

decreased CD4-positive, alpha-beta T cell number ( J:111874 )

• mice have lower percentage of single positive CD4+ T cells (3%) in the thymus compared to other genotypes

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:111874