Phenotypes Associated with This Genotype

Genotype
MGI:3706514

• Allelic Composition: Tg(Ins2-Nos2)40Okam/0
• Genetic Background: involves: C57BL/6 * CD-1 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal pancreatic islet morphology ( J:45520 )

• transgenic islet cells show extensive DNA strand breaks compared to control islets

decreased pancreatic beta cell mass ( J:45520 )

• proportion of insulin-producing cell mass to total pancreatic cell mass is markedly reduced at 4 weeks of age

• however, no infiltration of macrophages or lymphocytes (insulitis) is observed

homeostasis/metabolism

hyperglycemia ( J:45520 )

• mice show hyperglycemia by one week of age with blood glucose above 400 mg/dl

decreased circulating insulin level ( J:45520 )

• serum insulin level (0.53 ng/dl) is decreased compared to controls (3.68 ng/ml)

increased circulating ketone body level ( J:45520 )

• urine often tested positive for ketones

abnormal nitric oxide homeostasis ( J:45520 )

• nitrite levels in medium, indicative of NO release, increase in a time-dependent manner

• treatment with aminoguanidine (inhibitor of Nos2) delays elevation of blood glucose levels and reduction in pancreatic insulin contents; reduction in insulin producing cell mass is reduced or improved with treatment

increased urine glucose level ( J:45520 )

• urine glucose levels are 200-500 mg/dl by 1 week of age

renal/urinary system

increased urine glucose level ( J:45520 )

• urine glucose levels are 200-500 mg/dl by 1 week of age

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:4552