Phenotypes for Gopc MGI:3722133 MGI Mouse

asthenozoospermia ( J:78599 )

• at 20 weeks of age, sperm motility is significantly reduced (6.9% +/-4.4% compared to 59.3% +/-7.5% in control sperm)

• most motile sperm present display only sluggish movement

• defects in spermatogenesis are noted as early as step 2-3 spermatids, when fragmentation of acrosomal caps is already observed

abnormal sperm mitochondrial sheath morphology ( J:78599 )

• an abnormal arrangement of mitochondria in the mitochondrial sheath is observed

coiled sperm flagellum ( J:78599 )

• some mutant epididymidal spermatozoa have their tails coiled around their nuclei; also observed in mature testicular spermatids

abnormal acrosome morphology ( J:78599 )

• early round spermatids exhibit fragmentation of the acrosomal cap and abnormal large vesicles caused by defective fusion of the Golgi-derived transport vesicles to the acrosome

• elongated spermatids and epididymal spermatozoa display prominent acrosome vacuolization, loss of adhesion to the nucleus, and total acrosome loss

globozoospermia ( J:78599 )

• all mutant epididymidal spermatozoa exhibit a round or ovoid head shape; also observed in mature testicular spermatids

absent acrosome ( J:78599 )

• in some cases, a complete loss of acrosomes is observed

detached acrosome ( J:78599 )

• loss of adhesion to the nucleus is often observed

abnormal sperm nucleus morphology ( J:78599 )

• all elongated spermatids and epididymal spermatozoa display malformed nuclei with a round or ovoid morphology

• occasional vacuolization or invagination give the nucleus a crescent-shaped structure

male infertility ( J:78599 )

• male homozygotes are sterile

impaired fertilization ( J:78599 )

• intracytoplasmic sperm injection (ICSI) of abnormal mutant sperm into oocytes results in cleavage into blastocysts only when injected oocytes are stimulated electrically 30 min after ICSI