Phenotypes Associated with This Genotype

Genotype
MGI:3759575

cx3

• Allelic Composition: Cdk2^tm1Sgo/Cdk2^tm1Sgo; Cdk4^tm2.1Bbd/Cdk4^tm2.1Bbd
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:125217 )

• although mice move and ingest milk at birth, they die within 24 hours due to cardiac failure

cardiovascular system

increased myocardial fiber size ( J:125217 )

• in some cases cardiomyocytes are hypertrophic and are partially disorganized by prominent capillaries

thin ventricular wall ( J:125217 )

• thin ventricular walls are associated with a one-third decrease in the number of proliferating cardiomyocytes

abnormal fetal cardiomyocyte proliferation ( J:125217 )

• thin ventricular walls are associated with a one-third decrease in the number of proliferating cardiomyocytes

congestive heart failure ( J:125217 )

• mice die within 24 hours of birth due to cardiac failure

liver/biliary system

liver hypoplasia ( J:125217 )

• fetal livers contain 60% of the cells found in wild-type livers

• however, this reduction is proportional to the reduction in overall size of embryos

• the number of hematopoietic cells in the fetal liver is reduced compared to in wild-type mice

abnormal liver physiology ( J:125217 )

• mice suffer from congestive livers

hematopoietic system

abnormal definitive hematopoiesis ( J:125217 )

• the number of hematopoietic cells in the fetal liver is reduced compared to in wild-type mice

• however, the number of granulocyte-macrophage progenitors, common myeloid progenitors and megakaryocyte-erythroid progenitor cells are normal

decreased erythrocyte cell number ( J:125217 )

• at E17.5, mice have a small decrease in red blood cell (RBC) number (1.27x10⁶+/-0.14 RBC per mm³ compared to 1.45x10⁶+/-0.11 RBC per mm³ in wild-type mice)

• however, this decrease does not affect viability

cellular

abnormal fetal cardiomyocyte proliferation ( J:125217 )

• thin ventricular walls are associated with a one-third decrease in the number of proliferating cardiomyocytes

abnormal cell cycle ( J:125217 )

• primary mouse embryonic fibroblast cells are delayed in the percent of cells entering into S phase but eventually equalize with wild-type cells

decreased fibroblast proliferation ( J:125217 )

• proliferation of mouse embryonic fibroblast (MEF) cells is decreased relative to wild-type MEFs

growth/size/body

decreased body weight ( J:125217 )

• at birth mice weigh 25% to 40% less than wild-type mice

muscle

increased myocardial fiber size ( J:125217 )

• in some cases cardiomyocytes are hypertrophic and are partially disorganized by prominent capillaries

abnormal fetal cardiomyocyte proliferation ( J:125217 )

• thin ventricular walls are associated with a one-third decrease in the number of proliferating cardiomyocytes