Phenotypes Associated with This Genotype

Genotype
MGI:3759748

cn1

• Allelic Composition: Ppp2r3c^tm1Imku/Ppp2r3c^tm1Imku; Tg(CD19-cre/ERT2)1Cgn/?
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased transitional stage B cell number ( J:100706 )

• the number of transitional-1 (IgM^highIgD^low) B cells is increased (5.38% compared to 3.5 % in heterozygotes)

increased pro-B cell number ( J:100706 )

• the number of pro-B cells (B220^lowCD43+) is high relative to in heterozygotes

decreased B cell number ( J:100706 )

• the number of splenic B cells is reduced to 60% of the number in heterozygotes

• mice have half the number of B220+ B cells as do heterozygous mice

• IgM+IgD+ recirculating B cells are decreased in number (to 7.04% from 13.4% in heterozygous mice)

• the proportion of B cell in the spleen is decreased (35.3% compared to 48.3% in heterozygotes)

• the number of IgD+ B cells in the extrafollicular regions of the spleen is decreased

decreased immature B cell number ( J:100706 )

• the number of pre-B cells and immature B cells (B220^lowCD43-) is decreased from 31% to 22.7% of control B cells

decreased transitional stage B cell number ( J:100706 )

• the number of transitional-2 (IgM^highIgD^high) B cells is decreased (7.08% compared to 9.88% in heterozygotes)

decreased mature B cell number ( J:100706 )

• the number of mature B cells (B220^highCD43-) is 2.72% (50% of the number in heterozygous mice)

• the number of IgM^lowIgD^high mature B cells is decreased (20.8% compared to 31.3% in heterozygotes)

• the proportion of mature B cells is decreased in the auxillary lymph nodes (9.33% compared to 16.4% in heterozyotes) and peripheral blood (18.1% compared to 51.3% in heterozygotes)

decreased B-1a cell number ( J:100706 )

• the number of B-1a cells is decreased (10.5% compared to 17.2% in heterozygotes)

decreased follicular B cell number ( J:100706 )

• the number of mature follicle B cells is decreased (27.1% compared to 40% in heterozygotes)

decreased pre-B cell number ( J:100706 )

increased T cell number ( J:100706 )

• the proportion of mature T cells in the spleen is increased (49.9% compared to 39% in heterozygotes)

small spleen ( J:100706 )

decreased spleen germinal center size ( J:100706 )

• after immunization with sheep red blood cells initiates small germinal centers relative to in heterozygotes

abnormal B cell activation ( J:100706 )

• following B cell receptor stimulation, B cells exhibit increased apoptosis (33.8% compared to 15.3% in heterozygotes) that is caspase-3 independent, increased mitochondrial membrane depolarization (64% compared to 43% in heterozygotes), and more single and double-strand DNA breaks (in 32.2% of cells compared to 21.1% of cells from heterozygotes)

• however, the level of spontaneous apoptosis and response to LPS stimulation are normal

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

abnormal humoral immune response ( J:100706 )

• however, IgM response to TNP-Ficoll and TNP-keyhole limpet hemocyanin is normal

• IgG response to TNP-Ficoll and TNP-keyhole limpet hemocyanin is slightly decreased at 14 days after immunization

hematopoietic system

increased transitional stage B cell number ( J:100706 )

• the number of transitional-1 (IgM^highIgD^low) B cells is increased (5.38% compared to 3.5 % in heterozygotes)

increased pro-B cell number ( J:100706 )

• the number of pro-B cells (B220^lowCD43+) is high relative to in heterozygotes

decreased B cell number ( J:100706 )

• the number of splenic B cells is reduced to 60% of the number in heterozygotes

• mice have half the number of B220+ B cells as do heterozygous mice

• IgM+IgD+ recirculating B cells are decreased in number (to 7.04% from 13.4% in heterozygous mice)

• the proportion of B cell in the spleen is decreased (35.3% compared to 48.3% in heterozygotes)

• the number of IgD+ B cells in the extrafollicular regions of the spleen is decreased

decreased immature B cell number ( J:100706 )

• the number of pre-B cells and immature B cells (B220^lowCD43-) is decreased from 31% to 22.7% of control B cells

decreased transitional stage B cell number ( J:100706 )

• the number of transitional-2 (IgM^highIgD^high) B cells is decreased (7.08% compared to 9.88% in heterozygotes)

decreased mature B cell number ( J:100706 )

• the number of mature B cells (B220^highCD43-) is 2.72% (50% of the number in heterozygous mice)

• the number of IgM^lowIgD^high mature B cells is decreased (20.8% compared to 31.3% in heterozygotes)

• the proportion of mature B cells is decreased in the auxillary lymph nodes (9.33% compared to 16.4% in heterozyotes) and peripheral blood (18.1% compared to 51.3% in heterozygotes)

decreased B-1a cell number ( J:100706 )

• the number of B-1a cells is decreased (10.5% compared to 17.2% in heterozygotes)

decreased follicular B cell number ( J:100706 )

• the number of mature follicle B cells is decreased (27.1% compared to 40% in heterozygotes)

decreased pre-B cell number ( J:100706 )

increased T cell number ( J:100706 )

• the proportion of mature T cells in the spleen is increased (49.9% compared to 39% in heterozygotes)

small spleen ( J:100706 )

decreased spleen germinal center size ( J:100706 )

• after immunization with sheep red blood cells initiates small germinal centers relative to in heterozygotes

abnormal B cell activation ( J:100706 )

• following B cell receptor stimulation, B cells exhibit increased apoptosis (33.8% compared to 15.3% in heterozygotes) that is caspase-3 independent, increased mitochondrial membrane depolarization (64% compared to 43% in heterozygotes), and more single and double-strand DNA breaks (in 32.2% of cells compared to 21.1% of cells from heterozygotes)

• however, the level of spontaneous apoptosis and response to LPS stimulation are normal

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal

cellular

decreased B cell proliferation ( J:100706 )

• B cell proliferation cannot be induced by B cell receptor cross-linking with or without IL-4

• however, B cell proliferation in response to LPS is normal