Phenotypes Associated with This Genotype

Genotype
MGI:3764997

cx2

• Allelic Composition: Enah^tm1Fbg/Enah^tm1Fbg; Evl^tm1Fbg/Evl^tm1Fbg; Vasp^tm1Ref/Vasp^tm1Ref
• Genetic Background: involves: 129 * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:127447 )

• mice die between E16.5 and P0 with a number of defects including intraamniotic hemorrhage, hydrops fetalis and frequent exencephaly

nervous system

abnormal radial glial cell morphology ( J:127447 )

• aberrant extensions of radial processes into ectopic growths are observed

abnormal cortical intermediate zone morphology ( J:127447 )

• mice lack uDTI staining in the intermediate zone (IZ) indicating an absence of organized axon tracts

• the IZ is thinner than in wild-type mice

• IZ cortices are nearly devoid of neurofilament protein + axons Tau-1 staining is reduced

abnormal cortical plate morphology ( J:127447 )

• the cortices show cortical neurons pouring through gaps in the pial membrane and spreading tangentially within the subarachnoid space compared to the well organized cortical plate and intact pial membrane in wild-type mice

• however, laminin staining surrounding ectopias is normal and in regions without ectopias cortical plate architecture is preserved

hydrocephaly ( J:127447 )

• mice display hydrocephalus ex vacuo

enlarged brain ventricles ( J:127447 )

• at E18.5, mice display enlarged ventricle size

abnormal brain white matter morphology ( J:127447 )

• all cortical fiber tracts are missing from the brain, including all major forebrain commissures and the internal capsule

absent brain internal capsule ( J:127447 )

abnormal brain pia mater morphology ( J:127447 )

• gaps in the pial membrane allow spread of cortical neurons into the subarachnoid space

abnormal subarachnoid space morphology ( J:127447 )

• contains ectopic cortical neurons

exencephaly ( J:127447 )

• between E16.5 and P0, mice frequently display exenchephaly

abnormal neurite morphology ( J:127447 )

• after 48 hours in culture, 74% of neuritis are at stage 2 of neuritogenesis with only 2% at stage 3 compared to 10% of wild-type cells at stage 1 and 69% at stage 3

• however, axonal polarization is normal

ectopic cortical neuron ( J:127447 )

• at E18.5, mice exhibit cortical ectopias

homeostasis/metabolism

hydrops fetalis ( J:127447 )

• between E16.5 and P0

cardiovascular system

hemorrhage ( J:127447 )

• between E16.5 and P0 mice exhibit intraaminotic hemorrhage

cellular

abnormal radial glial cell morphology ( J:127447 )

• aberrant extensions of radial processes into ectopic growths are observed