Phenotypes for Nog MGI:3819135 MGI Mouse

abnormal neural tube morphology ( J:118341 )

incomplete rostral neuropore closure ( J:118341 )

• at E11.5 embryos show open brain phenotype

open neural tube ( J:118341 )

• at E11.5 caudal neural tube is open

abnormal notochord morphology ( J:118341 )

• abnormal at E8.5 and E9, indicating delayed detachment from dorsal foregut endoderm; appears hypertrophic compared to wild-type at E9

• no clear boundary between notochord and endoderm is detected at E8.5

• at E9.5, notochord has lateral branches close to or tethered to dorsal foregut in contrast to wild-type notochord

• increased apoptotic cell numbers are seen at E9.5-E9.75

• non-notochordal cells are observed within the notochord at E9.0

abnormal esophagus development ( J:118341 )

• at E10.5-11.5 most mutants display Type C esophageal atresia/tracheoesophageal fistula (EA/TEF); some embryos show milder phenotype indicative of esophageal stenosis

abnormal foregut morphology ( J:118341 )

• at E9.5, reduction in dorsoventral diameter of the foregut is observed; dorsal foregut endoderm is reduced in mutant embryos

• loosening or loss of dorsal foregut cells is consistently observed at E9; endodermal cells show basement membrane disruption

esophageal atresia ( J:118341 )

• at E10.5-11.5, >80% of embryos display Type C esophageal atresia/tracheoesophageal fistula (EA/TEF)

esophagus stenosis ( J:118341 )

• severe narrowing of the esophagus is seen by E9.5

tracheoesophageal fistula ( J:118341 )

• at E10.5-11.5, >80% of embryos display Type C esophageal atresia/tracheoesophageal fistula (EA/TEF) with the upper esophagus ending in a blind pouch and the lower esophagus connects to the trachea via a fistula