Phenotypes Associated with This Genotype

Genotype
MGI:4361657

cn12

• Allelic Composition: Amhr2^tm3(cre)Bhr/Amhr2⁺; Nr2f2^tm2.1Tsa/Nr2f2^tm2.1Tsa
• Genetic Background: involves: 129S7/SvEvBrd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, incomplete penetrance ( J:120852 )

• only ~7% of mutant mice are born, indicating embryonic lethality probably due to vascular and heart anomalies resulting from ectopic cre expression

embryo

abnormal placental labyrinth vasculature morphology ( J:120852 )

• by day 10 of pregnancy, formation of fetal blood vessels from the allantois is clearly absent

increased trophoblast giant cell number ( J:120852 )

• at day 9 of pregnancy, but nor earlier, secondary TGC differentiation appears to increase because more cells are present

• by day 10 of pregnancy, many layers of secondary TGC are noted in mutant females relative to control animals

abnormal maternal decidual layer morphology ( J:120852 )

♀ • at day 9 of pregnancy, the mutant uterus displays a reduced number of decidual sites with the absence of embryo or the presence of growth-retarded embryos

decreased spongiotrophoblast cell number ( J:120852 )

• by day 10 of pregnancy, mutant placentas show a reduction in spongiotrophoblast cell number relative to wild-type placentas

absent placental labyrinth ( J:120852 )

• by day 10 of pregnancy, the mutant labyrinth fails to develop

abnormal placenta development ( J:120852 )

• at day 9 of pregnancy, but nor earlier, abnormal placenta development and frequent hemorrhages are observed

• by day 10 of pregnancy, the mutant labyrinth fails to develop

reproductive system

vaginal hemorrhage ( J:120852 )

♀ • mutant females display vaginal bleeding 10-12 days after the presence of a copulatory plug

abnormal maternal decidual layer morphology ( J:120852 )

♀ • at day 9 of pregnancy, the mutant uterus displays a reduced number of decidual sites with the absence of embryo or the presence of growth-retarded embryos

abnormal myometrium morphology ( J:120852 )

♀ • the circular smooth muscle layer of mutant uteri appears disorganized relative to that of wild-type uteri, as shown by anti-smooth muscle actin immunostaining

♀ • in contrast, the mutant endometrium appears relatively normal

short uterine horn ( J:120852 )

♀ • mutant uterine horns appear shorter than those of control mice

impaired embryo implantation ( J:120852 )

♀ • upon mating with wild-type males, mutant females exhibit a significant lower number of implantation sites per pregnant female than control females (5.3 2.4 vs 8.8 2.2, respectively)

increased miscarriage rate ( J:120852 )

♀ • upon mating with wild-type males, mutant females display vaginal bleeding 10-12 days after the presence of a copulatory plug, indicating failure to maintain pregnancy

♀ • at day 9 of pregnancy, most embryos are reabsorbed and a few are growth-retarded

♀ • however, production of endogenous estradiol and progesterone remains unaffected in mutant females

abnormal uterine environment ( J:120852 )

♀ • despite normal ovarian histology and function, mutant female mice receiving a wild-type ovary are unable to produce pups as observed before ovary transfer

♀ • only 1 of 6 mutant females with wild-type ovaries gave birth to one pup in a 6-month period whereas wild-type females carrying mutant ovaries exhibited normal litter sizes with pups derived from the donor ovary

reduced female fertility ( J:120852 )

♀

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:120852 )

• by day 10 of pregnancy, formation of fetal blood vessels from the allantois is clearly absent

hemorrhage ( J:120852 )

• mutant embryos with hemorrhage are observed

vaginal hemorrhage ( J:120852 )

♀ • mutant females display vaginal bleeding 10-12 days after the presence of a copulatory plug

homeostasis/metabolism

edema ( J:120852 )

• mutant embryos with edema are observed