Phenotypes Associated with This Genotype

Genotype
MGI:4420540

cx30

• Allelic Composition: Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj; Cdkn2a^tm1Rdp/Cdkn2a^tm1Rdp
• Genetic Background: involves: 129/Sv * 129S2/SvPas * C57BL/6 * C57BL/6J * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell physiology ( J:72780 )

• vHaras-induced foci formation in primary keratinocytes is increased compared with similarly treated wild-type and single homozygous cells indicating increased tumorgenicity

increased keratinocyte proliferation ( J:72780 )

• keratinocyte proliferation is increased compared to in wild-type cells

delayed cellular replicative senescence ( J:72780 )

• age-related senescence in the epidermis in vivo is almost absent compared to in wild-type mice

• vHaras-induced premature senescence of primary keratinocytes is decreased compared with similarly treated wild-type cells

• however, calcium- and Tgfb-induced cell growth arrest is normal

neoplasm

increased squamous cell carcinoma incidence ( J:72780 )

• 2 tumors are observed when keratinocytes are transplanted into nude mice unlike when wild-type cells are used

• all nude mice transplanted with keratinocytes infected with a vHaras-expressing retrovirus develop poorly differentiated SCCs with spindle cell morphology unlike nude mice receiving similarly treated wild-type cells

integument

abnormal skin development ( J:72780 )

• mice exhibit altered expression of several markers of epidermis differentiation compared with wild-type mice

abnormal skin physiology ( J:72780 )

• age-related senescence in the epidermis in vivo is almost absent compared to in wild-type mice

abnormal keratinocyte physiology ( J:72780 )

• as determined by marker expression, in vitro keratinocyte differentiation is altered compared with similarly treated cells from wild-type mice and single homozygotes

• vHaras-induced premature senescence of primary keratinocytes is decreased while foci formation is increased compared with similarly treated wild-type cells and single homozygous

• however, calcium- and Tgfb-induced cell growth arrest is normal

increased keratinocyte proliferation ( J:72780 )

• keratinocyte proliferation is increased compared to in wild-type cells