Phenotypes Associated with This Genotype

Genotype
MGI:5294956

• Allelic Composition: Ctf1^tm1Msd/Ctf1^tm1Msd
• Genetic Background: B6.Cg-Ctf1^tm1Msd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

obese ( J:176727 )

• mutants develop spontaneous adult-onset obesity, with weight gain first seen starting at 6 months of age

increased susceptibility to diet-induced obesity ( J:176727 )

• 2 month old mutants fed a high-fat diet for 12 weeks gain more weight and accumulate more epididymal, retroperitoneal, and subcutaneous fat mass than wild-type mice

adipose tissue

increased total body fat amount ( J:176727 )

• weight gain is mostly due to increased adipose tissue

decreased fat cell mitochondrial DNA content ( J:176727 )

• mitochondrial DNA levels are reduced in white adipose tissue, indicating impaired mitochondrial biogenesis

increased fat cell size ( J:176727 )

• adipocyte hypertrophy is seen in mature mutants

behavior/neurological

decreased food intake ( J:176727 )

• mutants become obese despite reduced food intake from 2-12 months compared to controls

homeostasis/metabolism

hyperglycemia ( J:176727 )

• hyperglycemia is seen from 6 months of age

increased circulating insulin level ( J:176727 )

• hyperinsulinemia is seen from 6 months of age

increased circulating leptin level ( J:176727 )

• increase in serum leptin levels in parallel with progression of weight gain

increased circulating cholesterol level ( J:176727 )

• hypercholesterolemia is seen from 6 months of age

decreased circulating free fatty acids level ( J:176727 )

• old obese mutants exhibit lower circulating free fatty acids than controls

decreased energy expenditure ( J:176727 )

• in 2 month old mutants, energy expenditure is reduced compared to wild-type mice, with differences increasing with age

increased susceptibility to diet-induced obesity ( J:176727 )

• 2 month old mutants fed a high-fat diet for 12 weeks gain more weight and accumulate more epididymal, retroperitoneal, and subcutaneous fat mass than wild-type mice

decreased oxygen consumption ( J:176727 )

• in 2 month old mutants, oxygen consumption is reduced compared to wild-type mice, with differences increasing with age

increased respiratory quotient ( J:176727 )

• respiratory quotient is elevated in mature obese mutants compared to mature wild-type mice

insulin resistance ( J:176727 )

• insulin resistance is seen in obese mutants

enhanced lipolysis ( J:176727 )

• adipocytes from obese mutants show increased baseline lipolysis and respond poorly to isoproterenol

endocrine/exocrine glands

increased pancreatic islet number ( J:176727 )

• pancreatic islet number is increased at 9 and 12 months of age

enlarged pancreatic islets ( J:176727 )

• pancreatic islet size is increased at 9 and 12 months of age

cellular

decreased fat cell mitochondrial DNA content ( J:176727 )

• mitochondrial DNA levels are reduced in white adipose tissue, indicating impaired mitochondrial biogenesis

abnormal aerobic respiration ( J:176727 )

• oxygen consumption is reduced in adipocytes from obese mutants

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
abdominal obesity-metabolic syndrome		DOID:0060611	OMIM:PS605552	J:176727
type 2 diabetes mellitus		DOID:9352	OMIM:125853 OMIM:601283 OMIM:601407 OMIM:603694 OMIM:608036	J:176727