Phenotypes for Atg7 Commd10 MGI:5295638 MGI Mouse

decreased common myeloid progenitor cell number ( J:176843 )

• reduction in most myeloid progenitors

• numbers of myeloid subsets CD11b⁺Gr1^- and CD11b^-Gr1⁺ are reduced in blood and decrease further over time

decreased bone marrow cell number ( J:176843 )

• overall bone marrow cell count is reduced

abnormal common lymphocyte progenitor cell morphology ( J:176843 )

• common lymphoid progenitors (Lin^-Flt3^HighIL7Ra^HighSca-1^Lowc-Kit^Low) are reduced in mutants

• numbers of CCR9⁺ lymphoid-primed multipotent progenitors are reduced in the bone marrow of mutants

abnormal hematopoietic stem cell morphology ( J:176843 )

• mutant LSK (Lin^-Sca-1⁺c-Kit⁺) cells accumulate mitochondria, mitochondrial superoxide, and DNA damage and exhibit increased levels of apoptosis and proliferation

decreased hematopoietic stem cell number ( J:176843 )

• numbers of hematopoietic stem cells are reduced in all mutants, regardless of disease progression

• absolute numbers of LSK (Lin^-Sca-1⁺c-Kit⁺) cells significantly increased in asymptomatic 7 week old mutants, however as they develop symptoms, the frequency of LSK cells falls to wild-type levels

• the Lin^-Sca-1^-c-Kit⁺ (LK) compartment, containing more mature hematopoietic progenitors, is decreased

abnormal leukocyte morphology ( J:176843 )

• numbers of CD11b⁺Gr1⁺ cells are increased in the blood at 5 and 6 weeks of age but then decrease to below wild-type levels at 8 weeks of age

• CD11b⁺Gr1⁺ cells in the spleen and bone marrow are increased and show higher proliferation rates

• mutants exhibit presence of atypical myeloid infiltrates in a wide range of organs

decreased leukocyte cell number ( J:176843 )

• mutants are cytopenic for all blood leukocyte populations except for CD11b⁺Gr1⁺ cells

decreased B cell number ( J:176843 )

• absolute cell counts of B cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

decreased NK cell number ( J:176843 )

• immature NK cells (Lin^-CD3^-CD122⁺NK1.1⁺DX5⁺) are depleted in the bone marrow

• absolute cell counts of NK cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

decreased T cell number ( J:176843 )

• absolute cell counts of T cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

abnormal hematopoietic stem cell physiology ( J:176843 )

• adult bone marrow cells from mutants transplanted together with CD45.1⁺ wild-type bone marrow cells into lethally irradiated wild-type hosts fail to contribute to reconstitution of cells in the hosts while in noncompetitive reconstitution experiments, lethally irradiated mice die within 4 weeks after transplantation with mutant bone marrow cells, indicating loss of hematopoietic stem cell activity