Phenotypes Associated with This Genotype

Genotype
MGI:5302204

• Allelic Composition: Crbn^tm1.2Jjh/Crbn^tm1.2Jjh
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:179427 , J:259276 )

N • mice exhibit normal basal locomotion and anxiety-like behaviors (J:179427)

N • mice exhibit normal social behavior in the three-chamber social interaction and social preference tests and no differences in repetitive grooming behavior (J:259276)

impaired contextual conditioning behavior ( J:179427 , J:259276 )

• mice freeze significantly less than control mice in a contextual fear conditioning task, indicating deficit it associative learning (J:179427)

• in a test for contextual fear memory, mice freeze less than wild-type mice (J:259276)

• treatment with Compound C, rescues the contextual fear memory defect (J:259276)

impaired spatial learning ( J:259276 )

• in the Morris water maze, mice take longer to locate the submerged platform during training indicating a deficit in learning

• in the water-based Y-maze task, mice take longer to locate the platform during training

• treatment with Compound C, a specific inhibitor of AMPK, rescues the learning deficits seen in the Y maze

abnormal long-term spatial reference memory ( J:259276 )

• mice do not spend more time in the goal zone as seen in wild-type mice at 48 hours following the final training session in the Morris water maze, indicating impaired long-term memory

• during the memory test of the Y-maze, mice take longer to locate the platform, indicating impaired long-term memory

• however, no difference in percentage of spontaneous alternations is seen indicating normal working memory

• treatment with Compound C, a specific inhibitor of AMPK, rescues the memory deficits seen in the Y maze

nervous system

nervous system phenotype ( J:259276 )

N • brains have normal gross morphology at postnatal week 8

abnormal synapse morphology ( J:259276 )

• marker analysis indicates an imbalance between excitatory and inhibitory synapses with higher markers of presynaptic and postsynaptic glutamatergic spines but lower levels of postsynaptic glutamatergic proteins

reduced long-term potentiation ( J:259276 )

• mice exhibit a deficit in hippocampal long-term potentiation compared to wild-type mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autosomal recessive intellectual developmental disorder		DOID:0060308	OMIM:PS249500	J:259276