Phenotypes for Gata4 MGI:5441538 MGI Mouse

neonatal lethality, incomplete penetrance ( J:117367 )

• nearly half of mice die within 1 day of birth

abnormal lung morphology ( J:117367 )

• dilated distal airways and patchy thickening of mesenchyme in some newborn mice

abnormal lung vasculature morphology ( J:117367 )

• some mice exhibit reduced artery density in the proximal airways compared with wild-type mice

abnormal lung saccule morphology ( J:117367 )

• enlarged in some mice

dilated respiratory conducting tube ( J:117367 )

• dilated distal airways in 7 of 21 mice between P1 and P8, more pronounced in accessory and middle lobes of the right lung

abnormal lung vasculature morphology ( J:117367 )

• some mice exhibit reduced artery density in the proximal airways compared with wild-type mice

abnormal heart morphology ( J:117367 )

• fatal cardiac lesions in some newborn

atrioventricular septal defect ( J:117367 )

• in some newborn mice

ventricular septal defect ( J:117367 )

• in some newborn mice

abnormal diaphragm central tendon morphology ( J:117367 )

• 6 of 21 mice exhibit an abnormal fusion of the central tendon to the liver surface with disorganized collagen bundles unlike in wild-type mice

diaphragmatic hernia ( J:117367 )

• in the ventral midline of some neonatal and adult mice with portions of the hernia sac near but not adherent to the liver contains a mixture of hepatocytes and connective tissue cells

• 3 of 21 mice exhibit overt herniation

increased apoptosis ( J:117367 )

• increased apoptosis without an affect on cell proliferation in amuscular diaphragm at E13.5

abnormal diaphragm central tendon morphology ( J:117367 )

• 6 of 21 mice exhibit an abnormal fusion of the central tendon to the liver surface with disorganized collagen bundles unlike in wild-type mice

reproductive system phenotype ( J:173711 )

N	• young adult females are fertile with no significant differences in average litter size, frequency of parturition, or ovarian follicular development relative to wild-type controls

small ovary ( J:173711 )

• ovaries of eCG-stimulated immature females are significantly smaller than eCG-treated wild-type ovaries

decreased ovary weight ( J:173711 )

• in response to exogenous gonadotropins, ovaries weigh significantly less than gonadotropin-stimulated wild-type ovaries

• however, unstimulated ovaries exhibit normal weight

abnormal ovary physiology ( J:173711 )

• ovaries of gonadotropin-stimulated immature females are significantly smaller, release fewer oocytes, produce less estrogen, and exhibit significantly lower mRNA levels of the steroidogenic genes Star, Cyp11a1, and Cyp19a1 than gonadotropin-stimulated wild-type ovaries

• however, basal ovarian levels of mRNA for Star, Cyp11a1 and Cyp19a1 are normal

abnormal endometrium morphology ( J:173711 )

• following eCG stimulation, uteri appear to be hypoestrogenic: glandular elements of the endometrial layer appear less complex, and only scattered glands are found in the stroma

decreased uterus weight ( J:173711 )

• uteri of eCG-stimulated immature females weigh significantly less than eCG-treated wild-type uteri

delayed sexual maturation ( J:173711 )

• females exhibit significantly delayed puberty, as indicated by a delay in the onset of estrous cyclicity

• onset of vaginal cornification is moderately but not significantly delayed

• delay in puberty is not due to impaired growth

delayed estrous cycle ( J:173711 )

• onset of estrous cyclicity is delayed an average of 12 days relative to wild-type females

• however, average cycle length and the proportion of time spent in any stage of the estrous cycle remain normal

decreased superovulation rate ( J:173711 )

• in response to exogenous gonadotropins, immature females release significantly fewer oocytes into the oviducts than superovulated wild-type controls

decreased circulating estradiol level ( J:173711 )

• following eCG stimulation, serum estradiol (E2) levels are significantly lower than in eCG-treated wild-type females

• however, basal serum E2 levels are relatively normal