Phenotypes Associated with This Genotype

Genotype
MGI:5460862

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Notch1)Dam}/Gt(ROSA)26Sor⁺; Tg(Neurog3-cre)C1Able/0
• Genetic Background: involves: 129S4/SvJaeSor

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:190530 )

• pups lose weight, become hyperglycemic, and die by postnatal day 3 (P3)

growth/size/body

decreased body weight ( J:190530 )

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:190530 )

N • endocrine differentiation is not completely abrogated, as some chromogranin A stained cells expressing EGFP are detected; some endocrine cells express PPY, with a few expressing glucagon or coexpressing PYY, but no insulin-, somatostatin-, or PP-expressing cells are observed

abnormal pancreatic duct morphology ( J:190530 )

• in contrast to normal pancreata where Neurog3-positive cells generate individual or pairs of duct cells, most EGFP-labeled cells are duct cells with Notch activation in 2-day old mice; no cells expressing both duct and endocrine markers are detected

• total numbers of duct cells are comparable to controls, but with Notch-activation, the fraction fated to duct lineage is substantially increased relative to cells fated to endocrine lineage

absent pancreatic islets ( J:190530 )

abnormal pancreas development ( J:190530 )

• islets fail to develop

digestive/alimentary system

abnormal pancreatic duct morphology ( J:190530 )

• in contrast to normal pancreata where Neurog3-positive cells generate individual or pairs of duct cells, most EGFP-labeled cells are duct cells with Notch activation in 2-day old mice; no cells expressing both duct and endocrine markers are detected

• total numbers of duct cells are comparable to controls, but with Notch-activation, the fraction fated to duct lineage is substantially increased relative to cells fated to endocrine lineage

homeostasis/metabolism

hyperglycemia ( J:190530 )