Phenotypes for Crb1 Crb2 Tg(Chx10-EGFP/cre,-ALPP)2Clc MGI:5582824 MGI Mouse

abnormal retina morphology ( J:207895 )

• between E15.5 and E17.5, the adherens junctions are gradually lost in the neural retina

abnormal Muller cell morphology ( J:207895 )

• increase in the number of Sox9-positive Muller cells

increased amacrine cell number ( J:207895 )

• increase in the number of GABAergic amacrine cells and late born GlyT1 positive amacrine cells

abnormal retina bipolar cell morphology ( J:207895 )

• increase in the number of Chx10-positive bipolar cells

abnormal retina progenitor cell morphology ( J:207895 )

• increase in population of late progenitor cells and late born cells due to dysregulation of the cell cycle at E17.5

• between E15.5 and E17.5, the nuclei of the retinal progenitors show abnormal orientation

abnormal retina layer morphology ( J:207895 )

• aberrant layering in the retina is seen at 1 month of age, with a single inner plexiform layer, an abnormal thick ganglion cell layer and a second broad nuclear layer

• all retinal cell types are generated but a separate photoreceptor nuclear layer, inner and outer segment layer and outer plexiform layer are not formed

• cellular mislocalization of late born cells (rod photoreceptors, Muller cells, and bipolar cells) near the retinal pigment epithelium are seen at E13.5

abnormal retina photoreceptor layer morphology ( J:207895 )

• no distinct photoreceptor layer is formed at P5

increased retina rod cell number ( J:207895 )

• increase in the number of rods at P10

disorganized retina layers ( J:207895 )

• severe disorganization of the retina

abnormal retina outer limiting membrane morphology ( J:207895 )

• the outer limiting membrane is perturbed at E13.5

decreased total retina thickness ( J:207895 )

• retinal vasculature defects leading to the thinning of the retinas at 3-6 months of age

increased total retina thickness ( J:207895 )

• increase in retina thickness at P10 and P14 due to excessive proliferation of late-born retinal progenitor cells

retina degeneration ( J:207895 )

• retinas degenerate rapidly after 1 month

abnormal eye physiology ( J:207895 )

• from E15.5 onwards, the number of M-phase cells is increased in retinas

• proportion of retinal cells in G1 is reduced and proportion of cells in S and G2/M is increased at E17.5, however at P1 and P5, cells return to normal cell cycle

increased retina apoptosis ( J:207895 )

• increase in the number of apoptotic cells in the retinas at P10 and P14 (rod photoreceptors), and 3 months (bipolar cells)

• the number of apoptotic cells in the retina is increased at E13.5 and E17.5 onwards

abnormal electroretinogram waveform feature ( J:207895 )

• mice exhibit a greater reduction in amplitudes of electroretinogram responses at 1 month of age than single Crb2 conditional mutants, with both scotopic and photopic responses

• electroretinogram responses are below detection at 3 and 6 months of age