Phenotypes Associated with This Genotype

Genotype
MGI:5644499

• Allelic Composition: Tg(MMTV-PyVT)#Mul/0
• Genetic Background: B6.FVB-Tg(MMTV-PyVT)#Mul

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased mammary adenocarcinoma incidence ( J:147923 )

♀ • mice develop mammary carcinomas with 100% penetrance

♀ • primary tumors develop at varying ages, with the first masses seen between 90-120 days of age

♀ • tumors comprise primarily of solid, glandular and acinar forms and often contain cystic areas

increased metastatic potential ( J:147923 )

♀ • progression to pulmonary metastases is seen in more than 95% of mice more than 130 days of age

immune system

decreased B cell proliferation ( J:147923 )

♀ • B cells from mice with tumors show a reduction in proliferation in response to stimulation with ConA and LPS

decreased splenocyte proliferation ( J:147923 )

♀ • splenocytes from mice with tumor loads show reduced proliferative responses in response to mitogens

enlarged spleen ( J:147923 )

♀ • enlarged spleen in mice with tumors, with mice with the highest tumor burdens having the largest spleens

increased regulatory T cell number ( J:147923 )

♀ • percentage of tumor-infiltrating Treg cells is increased

abnormal splenic cell ratio ( J:147923 )

♀ • percentage of macrophage-like populations and myeloid cells in the spleen increase with increasing tumor load, with a corresponding decrease in the percentage of T cells

♀ • however, percentage of NKT cells in the spleen is similar to controls

increased splenocyte number ( J:147923 )

♀ • total number of splenocytes increases progressively with increasing tumor burden

abnormal T cell physiology ( J:147923 )

♀ • T cells from CD3/CD28-stimulated splenocytes from tumor-bearing mice produce lower levels of IFN-gamma

decreased T cell proliferation ( J:147923 )

♀ • T cells from mice with tumors show a reduction in proliferation in response to stimulation with ConA and LPS

abnormal circulating cytokine level ( J:147923 )

♀ • tumor-bearing mice show increased serum levels of pro-inflammatory cytokines MCP-1 and TNF-alpha

increased circulating tumor necrosis factor level ( J:147923 )

♀ • in tumor-bearing mice

abnormal lymph node morphology ( J:147923 )

♀ • total number of lymph node cells in tumor-draining lymph nodes increases progressively until a total tumor burden between 8 and 12 cm³ is reached, after which a decrease in lymph node cell numbers is seen

abnormal lymph node cell ratio ( J:147923 )

♀ • increase in the percentages of B cells within the tumor-draining lymph nodes of mice with extensive tumor loads

♀ • however, percentage of NKT cells in the tumor-draining lymph nodes is similar to controls

homeostasis/metabolism

abnormal circulating cytokine level ( J:147923 )

♀ • tumor-bearing mice show increased serum levels of pro-inflammatory cytokines MCP-1 and TNF-alpha

increased circulating tumor necrosis factor level ( J:147923 )

♀ • in tumor-bearing mice

hematopoietic system

decreased B cell proliferation ( J:147923 )

♀ • B cells from mice with tumors show a reduction in proliferation in response to stimulation with ConA and LPS

decreased splenocyte proliferation ( J:147923 )

♀ • splenocytes from mice with tumor loads show reduced proliferative responses in response to mitogens

enlarged spleen ( J:147923 )

♀ • enlarged spleen in mice with tumors, with mice with the highest tumor burdens having the largest spleens

increased regulatory T cell number ( J:147923 )

♀ • percentage of tumor-infiltrating Treg cells is increased

increased myeloid cell number ( J:147923 )

♀ • myeloid cells in the spleen are increased in tumor-bearing mice

abnormal splenic cell ratio ( J:147923 )

♀ • percentage of macrophage-like populations and myeloid cells in the spleen increase with increasing tumor load, with a corresponding decrease in the percentage of T cells

♀ • however, percentage of NKT cells in the spleen is similar to controls

increased splenocyte number ( J:147923 )

♀ • total number of splenocytes increases progressively with increasing tumor burden

abnormal T cell physiology ( J:147923 )

♀ • T cells from CD3/CD28-stimulated splenocytes from tumor-bearing mice produce lower levels of IFN-gamma

decreased T cell proliferation ( J:147923 )

♀ • T cells from mice with tumors show a reduction in proliferation in response to stimulation with ConA and LPS

cellular

decreased B cell proliferation ( J:147923 )

♀ • B cells from mice with tumors show a reduction in proliferation in response to stimulation with ConA and LPS

decreased T cell proliferation ( J:147923 )

♀ • T cells from mice with tumors show a reduction in proliferation in response to stimulation with ConA and LPS

decreased splenocyte proliferation ( J:147923 )

♀ • splenocytes from mice with tumor loads show reduced proliferative responses in response to mitogens

endocrine/exocrine glands

increased mammary adenocarcinoma incidence ( J:147923 )

♀ • mice develop mammary carcinomas with 100% penetrance

♀ • primary tumors develop at varying ages, with the first masses seen between 90-120 days of age

♀ • tumors comprise primarily of solid, glandular and acinar forms and often contain cystic areas

integument

increased mammary adenocarcinoma incidence ( J:147923 )

♀ • mice develop mammary carcinomas with 100% penetrance

♀ • primary tumors develop at varying ages, with the first masses seen between 90-120 days of age

♀ • tumors comprise primarily of solid, glandular and acinar forms and often contain cystic areas

growth/size/body

enlarged spleen ( J:147923 )

♀ • enlarged spleen in mice with tumors, with mice with the highest tumor burdens having the largest spleens

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
breast cancer		DOID:1612	OMIM:114480	J:147923